嗅鞘细胞与氯化锂联合修复成年大鼠急性脊髓损伤的实验研究  

Study of combination therapy with olfactory ensheating cells and lithium chloride following adult rat acute spinal cord injury

作　　者：王路[1] 王吉兴[1] 闫慧博[1] 李凌霞[2] 鲁凯伍[1] 

机构地区：[1]南方医科大学南方医院脊柱骨病外科 [2]南方医科大学药理研究所,广州510515

出　　处：《神经解剖学杂志》2009年第3期317-322,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(No.30471761);广东省自然科学基金(No.04020415)资助项目

摘　　要：为观察联合应用氯化锂(LiCl)后,嗅鞘细胞(OECs)移植对大鼠急性脊髓损伤修复效果,并探讨二者之间可能的相互作用,本研究利用多中心急性脊髓损伤打击器(MASCIS impactor)制作大鼠脊髓打击伤模型,然后随机分为OECs组、LiCl组、OECs+LiCl组及空白对照组。分期用实验性脊髓损伤运动功能BBB评分法对后肢运动功能评分,并进行组织学检查,评价及比较各组修复效果。结果显示:OECs组与OECs+LiCl组BBB评分较LiCl组与对照组在2周后各时段有显著性差异(P<0.01);OECs+LiCl组BBB评分在4周后显著高于OECs组(P<0.01);组织学检查观察到OECs组与OECs+LiCl组的组织学改变与LiCl组和对照组有显著性差异,而且OECs+LiCl组相对于OECs组表现出更明显的促神经纤维再生。以上结果提示OECs对脊髓损伤的修复作用在联合LiCl后更加显著,表明二者具有良好的协同作用关系。To observe the repairing effect of olfactory ensheating cells（OECs） combined with lithium chloride （LiCl） on acute rat spinal cord injury and investigate the synergetic effect, we applied the MASCIS （multi-center acute spinal cord injury system） impactor to make the rats＇ spinal cord injury （SCI） models and divide into 4 groups randomly： OECs group, LiCl group, OECs ＋ LiCl group and control group. The Basso-Beattie-Bresnahan （BBB） hindlimb motor function score and histological examination were carried out in phases to evaluate the repair effects. The BBB score showed that OECs group and OECs ＋ LiCl group were more improved than LiCl group and control group after 2 weeks following the SCI, and there were significant differences （P 〈0.01 ） ; BBB score of OECs ＋ LiCl group after 4 weeks was significantly higher than OECs group （P 〈 O. 01 ）. Comparing to the LiCl group and the control group, the histological examinations of OECs group and OECs ＋ LiCl group were much better, OECs ＋ LiCI group was better than other 3 groups in promote the neural fiber regeneration. The present results suggest it is effective for functional and structural recovery of acute SCI adopting combined therapy with OECs and LiCl.

关 键 词：嗅鞘细胞 氯化锂 脊髓损伤 BBB评分 大鼠 

分 类 号：R651.2[医药卫生—外科学]