尿路感染生物被膜菌临床分布及耐药性研究  

Research on clinical distribution and antimicrobial resistance to bacterial biofilm in urinary tract infections

作　　者：邹彬彬[1] 漆涌[1] 伍勇[1] 

机构地区：[1]中南大学湘雅三医院检验科,长沙410013

出　　处：《国际检验医学杂志》2009年第5期461-463,共3页International Journal of Laboratory Medicine

摘　　要：目的筛选尿路感染生物被膜菌,了解其临床分布,进而通过生物被膜菌与浮游菌耐药差异性分析,探讨细菌生物被膜对抗生素的耐药特性,以及初探生物被膜菌在体内较"真实"的耐药性。方法收集120例导尿管相关尿路感染(UTIs)患者导尿管和尿液标本,筛选生物被膜菌株和相应浮游株,对生物被膜半定量检测,药敏实验分析生物被膜菌株和相应浮游菌在普通MH培养基上耐药性差异以及生物被膜阳性菌在泊洛沙姆(Poloxamer,F-127)培养基和普通MH培养基上耐药差异性的分析。结果120例UTIs患者导尿管中筛选出生物被膜菌48株,阳性率为40%;生物被膜菌与相应的浮游菌在普通MH培养基上药敏结果相似,差异无统计学意义(P>0.05);生物被膜菌在Poloxamer培养基和MH培养基上药敏结果差异有统计学意义(P<0.05),且前者耐药性更强。结论本院尿路感染生物被膜菌在临床上以葡萄球菌属和肠球菌属为主;生物被膜菌与浮游菌在体外耐药性分析未见明显差异,推测Poloxamer培养基有可能表现生物被膜菌较真实的对药物的耐受情况,其耐受性更强。Objective To clarify the clinical distribution and antimicrobial resistance characteristic of bacterial biol.ilm during catheter-associated urinary tract infection, and to simulate biofil＇m ＂real state＂ in vivo. Methods Totally 120 patients with catheter-associated urinary tract infections （UTIs） were enrolled in the study. The urine specimens were collected for screening biofilm strains and the corresponding planktonic strains. The hiofilm was detected with semi quantitative detecting method. Antibiotics susceptihility test were performed on the biofilm bacteria to clarify the difference of drug resistance in common MH medium the between biofilm strains and the corresponding planktonic strains,as well as the difference of drug resistance of positive strains of the biofilm between Poloxamer medium and common MH medium. Results Totally 48 strains （48/120,40%） of biofilm bacteria were detected. The antibiotic susceptibility test of planktonic and biofilm bacteria in Mueller-Hinton agar showed no significant difference （P〉0.05）, while the antibiotic resistance of biofilm bacteria in Mueller-Hinton agar and Poloxamer hydrogel was statistically different （P〈0. 05）, and the former was stronger. Conclusion The biofilm bacteria during the urinary tract infection were mainly Staphylococci and Enteroeocci. The antimicrobial resistance of planktonic and biofilm bacteria have no significant difference in vitro. It is speculated that Poloxamer media may simulate the real living environment of biofilm bacteria and display their ＂true＂ drug resistance.

关 键 词：泌尿道感染 生物膜 抗药性 细菌 

分 类 号：R691.3[医药卫生—泌尿科学]