亚精胺浸种对玉米幼苗根系核蛋白体上结合态多胺含量的影响  

Effect of Seed Soaking with Spermidine on Contents of Polyamines Conjugated to the Deoxyribonucleic Acid-protein in Maize(Zea mays L.) Seedling Roots under Osmotic Stress

作　　者：周琳[1] 马克世[1] 杜红阳[2] 刘怀攀[1] 

机构地区：[1]周口师范学院生命科学系,河南周口466000 [2]河南农业大学作物生长与发育调控省重点实验室,河南郑州450002

出　　处：《种子》2009年第5期4-7,共4页Seed

基　　金：国家自然科学基金(No.30771296);河南省基础与前沿技术研究计划项目(No.082300430310)

摘　　要：用高压液相色谱(HPLC)方法检测了渗透胁迫下,玉米幼苗根系核蛋白体(deoxyribonucleic acid-protein:DNP)上的非共价结合态多胺(noncovalently conjugated polyamines:NCC-PAs)的含量。两个品种的NCC-Spd(亚精胺)和NCC-Spm(精胺)含量都因渗透胁迫而上升,而且抗旱性强的农大108上升幅度明显大于抗旱性弱的掖单13,在两个品种中未检测到NCC-Put(腐胺)。亚精胺浸种处理明显促进了渗透胁迫下掖单13核蛋白体上NCC-Spd和NCC-Spm含量的上升,也提高了掖单13幼苗对渗透胁迫的抗性。结果表明:亚精胺浸种处理,通过提高玉米幼苗根系核蛋白体上NCC-Spd和NCC-Spm的含量,从而增强玉米幼苗适应渗透胁迫的能力。The contents of noncovalently conjugated polyamines （NCC-PAs） to （DNP） isolated from maize （ Zea mays L.） seedling roots under osmotic stress deoxyribonucleic acid-protein were detected by HPLC. Resuits showed that after osmotic stress treatment for 7 d, the levels in NCC-spermidine （NCC-Spd） and NCC- spermine （NCC-Spm） of drought-tolerant Nongda 108 cv. increased more markedly than that of drought-sensitive Yedan 13 cv. , while the NCC-putrescine （NCC-Put） couldn＇t be statistically detected in two cultivars. Treatment of seed-soaking with Spd alleviated osmotic stress injury to Yedan 13 cv. seedlings, coupled with marked increases of NCC-Spd and NCC-Spm levels of this cuhivar. These results suggested that Spd seed-soaking could enhance the tolerance of maize seedlings, especially of the drought-sensitive cultivar, to osmotic stress, via increasing NCC-Spd and NCC-Spm in DNP of roots.

关 键 词：亚精胺浸种 玉米幼苗 核蛋白体(DNP) 结合态多胺 渗透胁迫 

分 类 号：Q945[生物学—植物学]