大鼠肝脏缺血-再灌注损伤时齐墩果酸对氧自由基的影响  

Effect of oleanolic acid on oxygen free radicals in liver ischemia-reperfusion injury in rats

作　　者：张杰[1] 许仄平[1] 孙宏斌[2] 姚爱华[3] 钱燕宁[1] 

机构地区：[1]南京医科大学第一附属医院麻醉科,210029 [2]中国药科大学新药研究中心 [3]南京医科大学第一附属医院肝脏外科,210029

出　　处：《临床麻醉学杂志》2009年第5期428-430,共3页Journal of Clinical Anesthesiology

基　　金：国家自然科学基金项目资助(30672523)

摘　　要：目的探讨齐墩果酸(OA)对大鼠肝脏缺血-再灌注损伤时氧自由基的影响。方法128只雄性SD大鼠随机分为假手术组(SH组)、缺血-再灌注组(IR组)、羧甲基纤维素钠(CMC-Na组)和OA组。建立70%肝脏缺血-再灌注模型,测定肝脏缺血60min再灌注0、3、6、12h后血清ALT活性和肝组织丙二醛(MDA)水平、超氧化物歧化酶(SOD)活性、谷胱甘肽(GSH)水平。结果再灌注3、6、12h,IR组、CMC-Na组、OA组血清ALT活性、肝组织MDA水平分别显著高于SH组(P<0.05),肝组织SOD活性、GSH水平分别明显低于SH组(P<0.05);OA组ALT活性、MDA水平分别较IR组和CMC-Na组明显降低(P<0.05),SOD活性和GSH水平分别较IR组和CMC-Na组显著升高(P<0.05)。结论OA对肝脏缺血-再灌注损伤具有一定保护作用,其抗肝脏缺血-再灌注损伤作用与抑制自由基的生成和释放有关。Objective To investigate the effects of oleanolic acid （OA） on oxygen free radicals in liver ischemia-reperfusion（IR） injury in rats. Methods One hundred and twenty-eight male SD rats were randomly divided into four groups of sham operation（SH）, IR, sodium carboxymethycellulose（CMC-Na）, and oleanolic acid （OA）. Rats I-R model was established by segmental （700//00） hepatic ischemia for 60 min, followed by reperfusion for different times. The animals were sacrificed at 0, 3, 6 or 12 h after reperfusion, respectively. The activity of serum ALT, content of malondialdehyde （MDA）, activity of superoxide dismutase（SOD） and content of glutathione （GSH） were measured. Results At 3 h, 6 h or 12 h after repel-fusion, the activity of ALT and the content of MDA were significantly higher in group IR, CMC-Na and OA than those in group SH and the levels of SOD and GSH were lower in group IR,CMC-Na and OA than those in group SH（P〈0. 05）. The activity of ALT and the content of MDA were significantly lower but SOD and GSH were higher in group OA than those in group IR and CMC-Na at 3 h, 6 h, or 12 after reperfusion（P〈0.05）. Conclusion OA can protect the liver from IR injury, which is related to the inhibition of production and release of oxygen free radicals.

关 键 词：齐墩果酸 肝脏缺血-再灌注损伤 氧自由基 

分 类 号：R285.5[医药卫生—中药学] R994.6[医药卫生—中医学]