氟比洛芬酯在大鼠手术切口组织中靶向分布的研究  

Target-distribution of flurbiprofen axetil in operative incision tissue in incision-induced rats

作　　者：陈雄刚[1] 陈百红[1] 林财珠[1] 

机构地区：[1]福建医科大学附属第一医院麻醉科,福州市350005

出　　处：《临床麻醉学杂志》2009年第5期431-432,共2页Journal of Clinical Anesthesiology

基　　金：福建省科技厅重点基金课题资助项目(2008Y0039);福建省卫生厅青年基金课题(2008-1-28)

摘　　要：目的探讨氟比洛芬酯在大鼠手术切口组织中靶向分布,并探讨其可能的作用机制。方法32只250g左右的SD大鼠随机分为四组,每组8只。K2、K4、K8、K16组分别注入氟比洛芬酯2、4、8、16mg/kg。使用反相高效液相法测定上清液中氟比洛芬的含量。结果各组手术侧大鼠足底肌肉组织中氟比洛芬浓度均较未手术侧大鼠足底肌肉组织中氟比洛芬浓度明显升高(P<0.01),K16组最高。各组随着氟比洛芬酯给药量的增加,手术侧大鼠足底肌肉组织中氟比洛芬浓度也随着增高(P<0.05或P<0.01);未手术侧大鼠足底肌肉组织中氟比洛芬浓度K2、K4、K8组两两组间比较无统计学意义,但K16组未手术侧大鼠足底肌肉组织中氟比洛芬浓度与K2、K4、K8组两两组间比较有升高(P<0.01)。结论氟比洛芬酯在手术切口组织中具有一定的靶向分布;静脉注射较高剂量(16mg/kg)的氟比洛芬酯时,大鼠正常肌肉组织中氟比洛芬的浓度显著升高。Objective To study target- distribution of flurbiprofen axetil in operative incision tissue in incision-induced rats. Methods Thirty-two- 250 g-weight rats were randomly divided into 4 groups. The incision pain model was established by being operated according to Brennan＇s method. Two hours after vena caudalis injection, all the rats were anesthetized deeply by pentobarbital sodium- perito injection 100 mg/kg,muscles of both hind paws were dissected, homogenated, centrifuged and supernatant fluids were dissociate. The concentration of flurbiprofen were detected by reversed phase high peforrnanee liquid chromatography（RP-HPLC）. Results In these groups of different dosage, the concentration of flurbiprofen in operative incision notably increased compared to that in the non- operative incision, especially in group K16. The concentration of flurbiprofen in operative incision of different dosage increased in dose-dependent manner. The difference of concentration of flurhiprofen in non-operative incisions of K2, K4, Ks was statistically insignificant, but the concentration of flurbiprofen in non-operative incision of K16 increased compared to that of K2, K4 and K8. Conclusion The distribution of flurbiprofen axeti in operative incision was targeted. When rats were injected flurbiprofen axetil at 16 mg/kg by vena caudalis, The concentration of flurbiprofen in the non- operative incision increased notably.

关 键 词：氟比洛芬酯 切口疼痛模型 反相高效液相法 

分 类 号：R614[医药卫生—麻醉学]