丙泊酚预处理对大鼠心肌缺血-再灌注损伤心肌细胞膜ATP酶活性的影响  

Effect of propofol preconditioning on activities of ATPase on myocardial cell membrane in rats with myocardial ischemia-reperfusion injury

作　　者：方向东[1,2] 张云云[1] 

机构地区：[1]合肥市第一人民医院麻醉科,230061 [2]合肥市妇幼保健院麻醉科

出　　处：《临床麻醉学杂志》2009年第5期433-434,共2页Journal of Clinical Anesthesiology

摘　　要：目的研究丙泊酚预处理对大鼠心肌缺血-再灌注损伤心肌细胞膜ATP酶活性及心肌组织形态学的影响。方法40只雄性SD大鼠随机分成五组,每组8只:对照组(C组),缺血-再灌注组(IR组),丙泊酚1组(P1组),丙泊酚2组(P2组),丙泊酚3组(P3组)。各组于再灌注60min后立即取左室心尖部心肌,检测心肌匀浆中心肌细胞膜Na+-K+-ATP、Ca2+-Mg2+-ATP酶活性;光镜下观察心肌组织形态学变化。结果IR时心肌细胞膜Na+-K+-ATP、Ca2+-Mg2+-ATP酶活性与C组相比明显下降(P<0.01);O1、P2、P3组心肌细胞膜Na+-K+-ATP、Ca2+-Mg2+-ATP酶活性与IR组相比明显升高,但仍低于C组(P<0.05)。结论丙泊酚可以减轻大鼠心肌缺血-再灌注损伤,其机制可能与恢复心肌细胞膜Na+-K+-ATP、Ca2+-Mg2+-ATP酶活性有关。Objective To investigate the effect of propofol preconditioning on activities of ATPase on myocardial cell membrane in rats with myocardial ischemia-reperfusion injury. Methods Forty Sprague-Dawley rats were subject to 30 min ischemia and 60 min reperfusion and randomly assigned to five group＇s, six rats per group： control （C） group; ischemia-reperfusion （IR） group propofol preconditioning group（P, according to the dosage of propofol, P was assigned to three groups： P1 , 10 ml·kg^-1 .h^-1 ; P2, 20 ml·kg^-1 ·h^1 ;P3, 30 ml·kg^-1 -h^-1 ）. The activities of Na^＋- K^＋-ATPase and Ca^2＋-Mg^2＋-ATPase of left ventricle myocardial tissue were measured by chemical colorimetric method. The myocardium pathomorphism was observed with light microscopes. Results The activities of Na^＋-K^＋-ATpase and Ca^2＋-Mg^2＋-ATPase in ischemia- reperfusion group were lower than control group and propofol preconditioning group（P〈0. 05 or P〈0. 01）. Conclusion Propofol preconditioning can produced cardioprotective effect against ischemia-reperfusion injury by recovering the ATPase activities of myocardial tissue.

关 键 词：丙泊酚 心肌再灌注损伤 预处理 ATP酶 

分 类 号：R96[医药卫生—药理学]