山东省亚急型克山病患儿与柯萨奇B组病毒的关系  

RELATIONSHIP BETWEEN SUBACUTE KESHAN DISEASES IN SHANGDONG POVINCE AND COXSACKIE VIRUS B

作　　者：张凤民[1] 张邻杰[2] 牛美娟[1] 周令望[2] 杨晓霞[3] 刘艺[2] 郭彩玲[1] 房维堂[3] 钟学宽[2] 

机构地区：[1]哈尔滨医科大学微生物教研室 [2]哈尔滨医科大学克山病研究所 [3]山东省地方病防治研究所

出　　处：《中国地方病防治》1998年第2期73-74,共2页Chinese Journal of Control of Endemic Diseases

基　　金：国家自然科学基金资助课题

摘　　要：为探讨山东省亚急型克山病患儿与柯萨奇B组病毒的关系,用缺口平移法制备生物素标记的COXB_3病毒的cDNA探针,与亚急型克山病病例尸检心肌组织9例及15例脑外伤,CO中毒等意外死亡的正常成人心肌组织和10例非病区7～8个月胎儿心组织切片进行原位杂交,结果9例山东亚急型克山病有5例检测到COXB组病毒的RNA,阳性率为55.5％(5/9),而对照组均为阴性。结果提示COXB组病毒感染是山东省亚急型克山病发病原因之一。In order to research the relationship between subacute Keshan disease and Coxsackie virus in Shandong province ,we labelled cox B3 probe with Biotin by nick translation and detected myocardial specimens from 9 patients with subacute Keshan disease, ISpatients with cerebral trauma, 10 patients with carbon monoxide poisoning and 10 fetus aged 7 ~ 8months from non - endemic areas by in situ hybridization. The results showed that of 9 patients with subacute Keshan diseases ,5(56% ) showed positive findings,but the control samples were negative. These results suggested that Cox B infection may be one of the causes of subacute Keshan disease in Shandong provice.

关 键 词：柯萨奇B组病毒 亚急型 克山病 原位杂交 儿童 

分 类 号：R725.423[医药卫生—儿科]