RP-HPLC测定骨健口服液中延胡索乙素的含量  

Determination of Tetrahydropalmatine in Gujian Oral Liquid by RP-HPLC

作　　者：潘云雪[1] 诸葛陇 张芳芳[2] 

机构地区：[1]杭州市药品检验所,杭州310017 [2]金华职业技术学院医学院,浙江金华321007

出　　处：《中国现代应用药学》2009年第5期400-402,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的建立骨健口服液中延胡索乙素的含量测定方法。方法采用反相高效液相色谱法。色谱柱:ZORBAX XDB-C18柱(150mm×4.6mm,5μm),流动相:0.1%磷酸溶液(用三乙胺调节pH值至6.0)-甲醇(45∶55),检测波长:280nm,流速:0.8mL·min-1,柱温:45℃。结果延胡索乙素在0.054～2.160μg内线性关系良好,相关系数r=0.9999,平均回收率为98.9%,RSD=0.5%(n=9)。结论该方法简便、准确、重现性好,可用于骨健口服液的质量控制。OBJECTIVE To determine the quantity of tetrahydropalmatine in Gujian oral liquid. METHODS RP-HPLC was used. Chromatographic conditions：ZORBAX XDB-C18 column （150 mm × 4.6 mm, 5 μm）, the mobile phase consisted of 0.1% H3PO4 （pH adjusted to 6.0 by triethylamine ）-methnol （ 45 ： 55 ）, the flow rate was 0.8 mL·min^-1, the detective wavelength was set at 280nm and column temperature was 45 ℃. RESULTS The calibration curve was linear in the range of 0.054-2.160 μg for the tetrahydropalmatine （r=-0.999 9）.The average recovery was 98.9% and the RSD was 0.5% （n=9）. CONCLUSION The method is simple and accurate with good repeatability, which may be used for the quality control of Gujian oral liquid.

关 键 词：反相高效液相色谱法 骨健口服液 延胡索乙素 

分 类 号：R917.101[医药卫生—药物分析学] R917.78[医药卫生—药学]