不同剂量激素对股骨近端的影响  

An experimental study on the effects of different dosages of glucocorticoid on proximal femurs in a rat model

作　　者：赵继军[1] 杨述华[1] 许伟华[1] 叶树楠[1] 王庆德[1] 何宇[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院骨科,武汉430022

出　　处：《中华实验外科杂志》2009年第6期712-714,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察不同剂量地塞米松对大鼠股骨近端组织学、骨形态发生蛋白-2（BMP-2）及其受体表达、细胞凋亡的影响。方法将96只雄性Wister大鼠随机分为对照组（C组）、地塞米松剂量5m/kg组（L组）、10mg／kg组（M组）、15m∥咤组（H组）。各组分别于用药后2、4、6、8周对股骨近端1／3骨质进行组织学、BMP4及其受体表达和细胞凋亡检测。结果实验组骨小梁／髓腔面积比、皮质／髓腔面积比、骨形态发生蛋白Ⅰ型受体（BMP—RⅠ）的含量均随用药时间的增加以及剂量的增加而降低，均低于C组。空骨陷窝计数和凋亡细胞计数则有随用药剂量及时间的增加而增加的趋势。L组的BMP-2平均染色面积百分比和BMP-2染色的平均吸光度在2周时明显高于对照组，而后降低，至8周时已明显低于对照组；M组和H组2周时接近正常水平，4、6、8周逐渐降低至较低水平。结论大剂量地塞米松可以显著降低股骨近端的骨质质量；激素对股骨近端的影响具有剂量依赖性。Objective To evaluate the effects of different dosages of dexamethasone on proximal femurs in a rat model by histology, expression of BMP-2 and its receptor, and apoptosis. Methods Ninety-six male rats were divided into 4 groups ： control group （ group C ）, 5 mg/kg dexamethasone group （ group L）, 10 mg/kg dexamethasone group （group M）, 15 mg/kg dexamethasone group （group H）. Drugs were administered twice a week by intramuscular injection. The histological changes, the expression of BMP-2 and its receptor and apoptosis of proximal femurs were observed. Results Area ratio of bone trabeculae and medullary cavity, area ratio of cortical bone and medullary cavity, contents of BMP-R I were reduced with time and increased dosages in experimental groups as compared with those in group C. The number of empty bone lacunae and the number of apoptosis cells were increased with time and increased dosages. Average percentage of dyed areas and average absorbance （A） values of BMP-2 in group L were markedly increased as compared with those in group C at 2nd week, and then decreased. On the 8th week ,they are significantly lower than in group C. While Average percentage of dyed areas and average A values of BMP-2 in groups M and H were at approximately the normal level at 2nd week, and decreased gradually at 4th, 6th and 8th week to a lower level. Conclusion High dosages of dexamethasone can damage the quality of proximal femur. Dexamethasone influces the proximal femur dose-deDendentlv.

关 键 词：糖皮质激素 股骨 骨形态发生蛋白-2 脱噬作用 

分 类 号：R686[医药卫生—骨科学]