胶原蛋白-硫酸肝素生物支架植入猪脑内凋亡相关蛋白的表达及意义  

The expression of apoptosis related proteins after collagen protein-heparan sulfate scaffold implanted into the brain tissues of pigs

作　　者：陈兴泳[1] 唐洲平[2] 唐荣华[2] 陈云[2] 孟祥武[2] 谢雪微[2] 李在望[2] 于加省[2] 王伟[2] 

机构地区：[1]福建省立医院第23区神经科,福州350001 [2]华中科技大学同济医学院附属同济医院临床神经研究中心

出　　处：《中华实验外科杂志》2009年第6期758-760,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30570628、30770751）;华中科技大学医工医理交叉基金资助项目

摘　　要：目的观察胶原蛋白-硫酸肝素生物支架植入猪脑内神经元凋亡及bax、bcl-2表达水平，探讨胶原蛋白-硫酸肝素支架在猪脑内生物相容性。方法清洁健康的体质量4～6kg的小猪20头，分实验组和对照组，每组10头。实验组通过微创手术方法将生物支架植入猪脑组织内，对照组操作方法类似，但不予植入支架。术后1、3、7、14、30d脑组织病理取材，TUNEL法检测凋亡细胞，SABC免疫组织化学方法检测bax、bcl-2蛋白表达水平。结果胶原蛋白-硫酸肝素支架成功植入猪脑内。术后1、3、7、14d两组脑组织均出现少量凋亡细胞，bax、bcl-2蛋白低水平表达，而在30天均无明显表达。实验组与对照组比较，在3、7d时期差异有统计学意义（P〈0．05）。结论胶原蛋白-硫酸肝素支架与猪脑组织具有良好的生物相容性，可用于中枢神经组织工程。Objective To investigate the effect of collagen protein-heparan sulfate scaffold im- plantation into the brain tissues of pigs on neuronal apoptosis and the expression of bax, bcl-2, and to disclose the biocompatibility of collagen protein-heparan sulfate scaffold with brain tissues of pigs in vivo. Methods Twenty clearing and healthy pigs weighing 4-6 kg were divided into two groups （ n = 10 each）. The collagen protein-heparan sulfate scaffold was implanted into pig＇ s cerebral tissues by micro-surgery. The control group was done similarly without implantation of the scaffold. The animals were sacrifriced at the 1 st,3rd ,7th, 14th and 30th day respectively after injury. Cell apoptosis was examined using the TUNEL assay,and the expression of bax and bcl-2 was detected using SABC immunohistochemistry. Results The collagen protein-heparan sulfate scaffold was well implanted into cerebral tissues. The short-term biocompatibility results showed a mild tissue response for all implant formulations. A small quantity of apoptotic cells emerged in the cerebral tissues of two groups at the lst,3rd,7th,and 14th day,meanwhile,the expression of bax and bcl-2 showed a low level. However, none of positive findings could be observed at the 30th day. There was significant difference between experimental and control groups at the 3rd and 7th day. Conclusion The scaffold has a good bioeompatibility with pig＇ s cerebral tissues, and can be used safely as biological materials in central neural tissue engineering.

关 键 词：胶原蛋白 硫酸肝素 生物相容性 猪 脱噬作用 

分 类 号：R686[医药卫生—骨科学]