CdSe/ZnS量子点探针用于检测猪链球菌2型溶菌酶释放蛋白(MRP)抗原的新方法研究  

A Novel Method to Detect Muramidase Released Protein(MRP) Antigen of Streptococcus sui Serotype 2 Based on CdSe/ZnS Quantum Dot Probe

作　　者：武红敏[1] 韩鹤友[1] 金梅林[2] 张安定[2] 

机构地区：[1]华中农业大学理学院农业微生物学国家重点实验室,武汉430070 [2]华中农业大学动物科技学院农业微生物学国家重点实验室,武汉430070

出　　处：《化学学报》2009年第10期1087-1092,共6页Acta Chimica Sinica

基　　金：国家自然科学基金(No.20675034);湖北省自然科学基金重点项目(No.2008CDA080);武汉市学科带头人计划(No.200851430484);国家973计划(No.2006CB504404)资助项目

摘　　要：合成了高发光效率的CdSe/ZnS量子点并制备了CdSe/ZnS量子点-溶菌酶释放蛋白(MRP)抗体探针,利用凝胶电泳和分子光谱法研究了MRP抗体与CdSe/ZnS量子点的结合机理.荧光光谱法优化了CdSe/ZnS量子点-MRP抗体探针制备的影响因素,建立了一种测定MRP抗原的新方法,其线性范围为5.0×10-8～1.5×10-6mol/L,线性相关系数为0.9976,检测限为1.9×10-8mol/L.High-quality CdSe/ZnS quantum dot and the CdSe/ZnS quantum dot-muramidase released protein （MRP） antibody probe were prepared, and the conjugation mechanism was studied according to the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE） and molecular spectrome- try. The factors influencing the preparation of CdSe/ZnS quantum dot-MRP antibody probe were optimized by fluorescence spectrometry. Under the optimum conditions, the probe showed good sensitivity to MRP antigen. The calibration graph was linear in the range from 5.0×10^-8 to 1.5×10^-6 mol/L of MRP antigen concentration, with a correlation coefficient of 0.9976 and a detection limit of 1.9×10^-8 mol/L.

关 键 词：猪链球菌2型 溶菌酶释放蛋白(MRP) CdSe/ZnS量子点探针 检测 

分 类 号：S854.43[农业科学—临床兽医学]