肾虚流产模型大鼠母胎界面CD80/CD86表达及中药调控研究  

Expression of CD80/CD86 in Maternal-fetal Interface of Abortion Rats with Kidney Deficiency and the Regulation of Chinese Medicine

作　　者：曾诚[1] 罗颂平[1] 李坤寅[1] 陶莉莉[1] 欧汝强 曾蕾[1] 何玉萍[1] 

机构地区：[1]广州中医药大学第一附属医院,广东广州510405 [2]广东省计划生育研究所,广东广州510600

出　　处：《新中医》2009年第6期105-107,共3页New Chinese Medicine

基　　金：广东省科技计划项目(编号:2007B031408002)

摘　　要：目的:研究肾虚流产模型大鼠蜕膜协同刺激分子(costimulatory molecules,CMs)CD80、CD86、CD28和细胞毒性T淋巴细胞相关抗原(CTLA-4)表达的变化以及助孕3号丸对妊娠免疫调节的作用与机理。方法:以羟基脲和米非司酮(RU486)灌胃建立肾虚流产大鼠模型。随机分为A(空白)组、B(模型)组、C(模型加西药)组、D(模型加助孕3号丸低剂量)组,E(模型加助孕3号丸中剂量)组,F(模型加助孕3号丸高剂量)组。收集子宫蜕膜采用流式细胞术测定CD80、CD86、CID28、CTLA-4表达值。结果:肾虚流产大鼠母胎界面CD80/CD86、CD28均呈高表达,CTLA-4呈低表达;治疗后与B组比较,C组和F组的CD80/CD86、CD28表达均下调,差异有显著性意义(P<0.05),CTLA-4表达上调,差异有显著性意义(P<0.05)。结论:助孕3号丸通过降调节CD80/CD86表达,使母胎界面细胞因子网络平衡向Th2型偏倚,降低母体免疫排斥,而达到防治流产的作用。Objective:To observe the expression of costimulatory molecules CD80,CD86 and CD28 as well as cytotoxic T lymphocyte associated antigen(CTLA-4) in uterine deciduae of abortion rats with kidney deficiency,and to explore the immunoregulatory effect and mechanism of Zhuyun Pill No.3(ZP3)on pregnancy.Methods:Kidney-deficiency abortion in rats was induced by gastric perfusion of hydroxyurea and mifepristone.The rats were randomized into 6 groups:blank group,model group,western medicine group,low-,middle- and high-dose ZP3 groups.The expression of CD80,CD86 and CD28 as well as CTLA-4 in uterine deciduae of rats was detected by flow cytometry.Results:Higher expression of CD80/CD86 and CD28,and lower expression of CTLA-4 were shown in maternal-fetal interface of abortion rats with kidney deficiency(P<0.05).After treatment,CD80/CD86 and CD28 expression in the model group were down-regulated, and CTLA-4 expression was up-regulated in western medicine group and high-dose ZP3 group(P<0.05).Conclusion:ZP3 has preventive and therapeutic effect on abortion through regulating CD80/CD86 expression and reducing maternal immunological rejection.

关 键 词：先兆流产 肾虚 助孕3号丸 协同刺激分子 大鼠 动物实验 

分 类 号：R714.21[医药卫生—妇产科学]