10个棉花品种的DNA多态性分析  

Analysis of Genetic Diversity among 10 Varieties of Cotton Based on RAPD Markers

作　　者：王建书[1] 朱美霞[1] 韩改英[1] 

机构地区：[1]河北工程大学农学院,河北邯郸056021

出　　处：《河南农业科学》2009年第5期37-39,共3页Journal of Henan Agricultural Sciences

基　　金：河北省教育厅科学研究计划资助项目(2006411)

摘　　要：采用RAPD技术,对10个棉花品种基因组进行DNA片断扩增,以研究品种间遗传多样性。结果表明:20个随机引物中,有8个引物能得到多态性条带。8个引物共扩增出96条带,其中33条多态性条带,多态率为34.4%。RAPD分析结果表明,用G06、G08、G11、G12、G17 5种引物可完全将这10个棉花品种区分开。此外,通过扩增BT基因发现,在非抗虫棉亚洲棉中也有BT基因的存在。The technique of random amplified polymorphic DNA（RAPD） was used to amplify ge- nomic DNA of 10 cotton varieties. A total of 96 bands were amplified with 8 polymorphic primers and the polymorphic rate was 34.4% （33/96）. The results showed that 5 kinds of random primers can differentiate the 10 varieties. In addition,there was also BT gene in G. arboreurn of non-resistant-pest cottons that was amplified BT gene.

关 键 词：棉花 随机扩增多态性DNA 遗传多样性 

分 类 号：S188[农业科学—农业基础科学] S562