小鼠胸腺Ghrelin阳性细胞的定位分布  

The Localization and Distribution of Ghrelin Positive Cells in Mouse Thymus

作　　者：张媛[1] 李英[1] 叶远兰[1] 马勇江[1] 李玉谷[1] 

机构地区：[1]华南农业大学兽医学院,广东广州510642

出　　处：《河南农业科学》2009年第5期122-124,共3页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(30871834)

摘　　要：以小鼠胸腺组织和原代培养的胸腺上皮细胞为研究材料,采用免疫组织化学和免疫荧光组织化学技术对Ghrelin阳性细胞在小鼠胸腺中的定位分布进行了研究。结果显示,小鼠胸腺中存在大量的Ghrelin免疫反应阳性细胞,主要分布在髓质,而皮质中只有少量呈零星分布的阳性细胞;这些阳性细胞为胸腺上皮细胞和巨噬细胞,其中不同表型的胸腺上皮细胞着色程度存在差异,表明Ghrelin是胸腺微环境的组成部分。To study the distribution of Ghrelin positive cells in mouse thymus,thymus gland tissue of mouse and primary cultural tbymic epithelial cell （TEC） were studied by using immunohistochemistry and immunofluorescence histochemistry technology in this study. The results showed that Ghrelin immunopositive cells were found in the cortex and medulla of thymus,especially centralized in thymic medulla. The matrix cell in the medulla, such as TEC and macrophage,were all positive cells and different in coloring among cells. These results suggested that Ghrelin was expressed in the thymus of mouse and shows little difference in TEC of different phenotype. So,Ghrelin would be participate in protecting the thymic mieroenvironment.

关 键 词：GHRELIN 胸腺 免疫组织化学 小鼠 

分 类 号：S852.1[农业科学—基础兽医学]