贵州乌蒙鸡血浆同工酶的多态性分析  

Analysis of Plasma Isozyme Polymorphism from Guizhou Wumeng Chicken

作　　者：张永亮[1] 简承松[2] 朱文适[2] 金深逊 李敬丹 

机构地区：[1]周口师范学院生命科学系,河南周口466000 [2]贵州大学生物技术学院动物科学系,贵州贵阳550025 [3]毕节地区畜牧兽医研究所,贵州毕节551700

出　　处：《河南农业科学》2009年第5期132-134,共3页Journal of Henan Agricultural Sciences

基　　金：贵州省"十五"攻关项目(200101139)

摘　　要：采用水平板聚丙烯酰胺凝胶电泳技术,检测分析了31份贵州乌蒙鸡血浆中的淀粉酶(Amy-1、Amy-2)、碱性磷酸酶(Akp-1、Akp-2)、酯酶-1(Es-1)同工酶的多态性。结果发现,除Amy-2和Akp-2位点呈单态外,其余位点均具有多态性,且akp-1、Akp-20、Amy-2i、Es-1C频率较高,Es-1A频率较低,Amy-1A和Amy-1B频率均在0.5000左右,Amy-1AB型频率很高。其多态位点百分比(P)和平均杂合度(H)均较高。这表明贵州乌蒙鸡血浆蛋白(酶)的多态程度较高,遗传多样性较为丰富,选择潜力较大,保种和开发利用价值较高。By using the horizontal polyacrylamide gel electrophoresis,the polymorphisms of 5 plasma isozymes isolated from 31 Guizhou Wumeng chickens were analyzed in this study. Except Akp - 2 and Amy - 2, the polymorphisms were found in Amy- 1,Akp- 1 and Es - 1. The gene frequencies of akp- 1,Akp- 2^o ,Amy- 2^i and Es- 1^c are higher than that of Es- 1^A. The frequencies of Amy- 1^A and Amy - 1^B are approximately 0. 500 0 ,respectively. The genotypic frequency of Amy- 1^AB is highest among all the genotypes detected in this experiment,and the percentages of polymorphic （P） and the average heterozygosity（H） are higher too. This indicated that the blood protein polymorphism of Wumeng chicken is high and the genetic diversity is wealthy,which means that the Guizhou Wumeng chicken has great potentialities for breeding and possesses great values of protection.

关 键 词：乌蒙鸡 同工酶 多态性 基因频率 平均杂合度 

分 类 号：S831.2[农业科学—畜牧学]