高效毛细管电泳法测定泰特中还原型和氧化型谷胱甘肽  

Determination of Reduced and Oxidized Glutathione in TAD by HighPerformance Capillary Electrophoresis

作　　者：徐志利[1] 邓兆勇[1] 杨仲元[1] 

机构地区：[1]广州市药品检验所

出　　处：《中国药科大学学报》1998年第1期49-51,共3页Journal of China Pharmaceutical University

摘　　要：用高效毛细管电泳法测定泰特中还原型和氧化型谷光甘肽含量。电泳系统由１００ｍｍｏｌ／Ｌ硼酸盐缓冲液和１０ｍｍｏｌ／ＬＮ－［２－羟基－１，１－二羟甲基－乙基］甘氨酸（ｔｒｉｃｉｎｅ）组成（ｐＨ８．５）；工作电压２０ｋＶ，检测波长２００ｎｍ，６ｍｉｎ内两组分达到基线分离。选用脲苷作内标物进行测定，两组分在２０～５００μｇ／ｍｌ范围内呈线性。A highperformance capillary electrophoresis(HPCE) method for the separation and determination of reduced and oxidized glutathione in TAD was developed. The run buffer contained 100 mmol/L borate and 10 mmol/L N[2hydroxy1,1bis(hydroxymethyl)ethyl] glycine(tricine) (pH 8.5), and the applied voltage was 20 kV with the detection wavelength of 200 nm. A baseline separation of the two types of glutathione could be achieved in 6 minutes. The standard curves were both linear from 20 to 500 μg/ml, using uridine as internal standard.

关 键 词：泰特 还原型 谷胱甘肽 氧化型 高效毛细管电泳 

分 类 号：R979.3[医药卫生—药品] R927.2[医药卫生—药学]