提高开胸结扎法建立猪心肌梗死模型成功率探讨  

Improve the successful rate of porcine myocardial infarction model produced by ligating coronary artery

作　　者：汤成春[1] 马根山[1] 黄峻[2] 杨国平[3] 张建桥[3] 

机构地区：[1]东南大学附属中大医院心内科,江苏南京210009 [2]南京医科大学第一附属医院心内科,江苏南京210029 [3]南京医科大学第一附属医院动物实验中心,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2009年第6期779-783,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生厅重大科研项目(H200209)

摘　　要：目的:探讨提高开胸结扎法建立猪急性心肌梗死模型成功率的方法。方法:幼猪16只,结扎组(10只)复合麻醉,机械通气,正中切口开胸结扎前降支中下1/3,术前、术中、术后静滴、术中浸润利多卡因;假结扎组(6只)穿线不结扎;心电图、饲养3周后冠状动脉造影、心肌核素显像检查、心脏标本HE及切片2,3,5-氯化三苯基四氮唑(TTC)染色。结果:结扎组手术成功率90%。心电图ST段明显抬高;3周后冠状动脉造影提示前降支中远端完全闭塞;核素心肌显像提示前壁、心尖部放射性稀疏或缺如;HE染色见均匀一致的纤维结缔组织增生;切片TTC染色不着色。结论:手术前后静滴及表面浸润利多卡因,正中切口开胸结扎冠状动脉前降支是建立猪心肌梗死模型有效的方法,可明显提高成功率。Objective：To study the success rate improvement of porcine myocardial infarction model produced by ligating coronary artery. Methods： 16 young farm pigs were devided into operated（n = 10） and sham-operated（n = 6） groups. Operated animals were anesthetized,incubated,mechanically ventilated and lidocaine injected intravenously. The heart was exposed by sterile median sternotomy and pericardiotomy,and the left anterior descending （LAD） was ligated from two third distal to its origin,lidocaine was intravenously injected before,during and after operation. Sham-Operated animals underwent a similar procedure except for the LAD ligation. The electrocardiogram （ECG） was monitored throught the entire operation,coronary angioplasty,positron emission tomography （PET）, triphenyltetrazolium chloride（TTC） staining and histopathological examination were performed 3 weeks later. Results：The success rat of porcine myocardial infarction model in the operated group was 90%. S-T elevation to single-phase curve appeared on the ECG after operation;coronary angiography showed that Medion and distal LAD was totally;PET demonstrated that radioactivity was sparse or absent in the left ventricular free wall and apex;light microscopy examination showed symmetrical fibrous connective tissue proliferation in the infracted area where the tissue is not stained by the TTC method. There were no abnormal results from macroscopic,ECG, PET and histopathological examination in sam-operated rats. Condution：LAD ligation by sterile median sternotomy is a available and feasible method to increase the success rate of porcine myocardial infarction model, lidocaine intravenously used.

关 键 词：猪 心肌梗死 结扎 利多卡因 成功率 

分 类 号：R541.4[医药卫生—心血管疾病]