高糖高脂饮食加链尿佐菌素建立实验性大鼠2型糖尿病模型  

Establishment of experimental rat model of type 2 diabetes with streptozotocin and high-glucose-high-fat diet

作　　者：孙晓芳 赵长勇 陈晖 王道荣 

机构地区：[1]扬州大学临床医学院内分泌科,江苏扬州225001 [2]扬州大学临床医学院普外科,江苏扬州225001

出　　处：《南京医科大学学报（自然科学版）》2009年第6期797-800,806,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省卫生厅指导性科研课题(Z200709)

摘　　要：目的:高糖高脂饮食加链尿佐菌素诱导实验性大鼠2型糖尿病模型的建立并观察稳定性。方法:60只SD雄性大鼠随机平均分为四组:对照组、链尿佐菌素(streptozotocin)组(STZ组)、高糖高脂饲养组(H.E组)和高糖高脂饲养+STZ组(H.E+STZ组)。STZ组和H.E+STZ组一次性腹腔内注射STZ35mg/kg。4周后监测体重、胰岛素、血糖、血脂、胰岛素敏感指数(ISI)和葡萄糖耐量实验曲线下面积(AUC)。结果:H.E组和H.E+STZ组大鼠空腹血浆胰岛素、血清甘油三酯(TG)、总胆固醇(TC)、ISI较对照组和STZ组增高(P<0.01);STZ组及H.E+STZ组大鼠空腹血糖、AUC较对照组和H.E组升高(P<0.01);注射STZ后4周H.E+STZ组空腹血糖、TG、TC、ISI较对照组和STZ组高(P<0.01)。结论:高糖高脂喂养能使大鼠胰岛素敏感指数明显下降,并且辅以小剂量链尿佐菌素后2型糖尿病大鼠模型成模率高、稳定性好、效果好、造模时间短。Objective：To establish the experimental rat model of type 2 diabetes by streptozotocin and high-glucose-high-fat diet and observe its stability. Methods： 60 SD male rats were randomly divided into four groups ： control group, streptozotocin group （STZ group）, high-glucose-high-fat feeding group （H.E group）and high-glucose-high-fat feeding＋STZ group （H.E＋STZ group）. STZ group and H.E ＋ STZ group were intraperitoneal injected with STZ 35 mg/kg. 4 weeks later,we measured and statistically analyzed the changes of body weight,blood insulin level,fasting blood glucose level,blood lipid level,insulin sensitivity index （ISI）and area under curve and insulin sensitivity index （AUC）of hamsters of every group at different time points. Results：The fasting plasma insulin, Serum triglyceride （TG）, total cholesterol （TC）, ISI of H.E group and H.E ＋STZ group significandy increased （P 〈 0.01 ）, the Fasting blood glucose and AUC of STZ group and H.E＋STZ group increased obviously （P 〈 0.01 ）. Compared with the control group and H.E group,the Fasting plasma insulin and TG,TC,ISI of H.E＋STZ group injected with STZ for four weeks increased significantly （P 〈 0.01）. Conclusion：High-glucose-high-fat diet significantly decreased insulin sensitivity index,and the type 2 diabetes rat models were established by high-glucose-high-fat diet combind with low dose injection of STZ with high success rate, effective stability and shorter model-making time.

关 键 词：高糖高脂饮食 链尿佐菌素 2型糖尿病 动物模型 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]