骨髓增生异常综合征骨髓组织中类胰酶阳性肥大细胞水平的研究  

The expression level of tryptase-positive mast cells in the bone marrow of MDS patients

作　　者：范娇[1] 张启国[1] 欧阳建[1] 吴鸿雁[2] 董海波[1] 

机构地区：[1]南京医科大学鼓楼临床医学院血液科,江苏南京210008 [2]南京医科大学鼓楼临床医学院病理科,江苏南京210008

出　　处：《南京医科大学学报（自然科学版）》2009年第6期807-810,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京市卫生局资助课题(YKK06086)

摘　　要：目的:探讨骨髓增生异常综合征(MDS)患者骨髓组织中类胰酶阳性的肥大细胞表达水平。方法:用骨髓塑料冷包埋技术结合免疫组织化学的方法检测10例健康供者,8例IDA患者、20例MDS(11例低危组,9例高危组)、17例急性髓性白血病(AML)患者骨髓类胰酶阳性的肥大细胞的数量,并作对比分析。结果:IDA组(63.43±44.29)与健康供者的类胰酶阳性肥大细胞数(61.43±56.19)类似(P值>0.05)。MDS组(195.95±117.39)较健康供者类胰酶阳性的肥大细胞数增多(P值<0.05)。MDS高危组(229.25±139.68)较低危组(171.73±98.07)高,但无统计学差异(P值>0.05));AML组(608.44±342.58)较MDS组明显增多(P值<0.01)。各组类胰酶阳性肥大细胞数与外周血常规、骨髓原始粒细胞数、嗜酸性粒细胞数、嗜碱性粒细胞数无相关性。结论:MDS和AML患者骨髓组织中均存在类胰酶阳性肥大细胞数目异常增多现象,它可能在MDS的病理发生发展中起一定作用。Objective：To explore the level of tryptase-positive mast cells in the bone marrow of MDS patients. Methods：Bone marrow biopsies from 10 healthy controls, 8 IDA,20 myelodysplastic syndrome and 17 acute myeloid leukemias were embedded in cold plastic resin. Immunohistochemical method was used to show the number of tryptase-positive cells.The level of tryptase-positive cells were compared. Results：No difference was found between the level of tryptase-positive mast cells in IDA group （63.43± 44.29） and healthy group（61.43 ± 56.19）（P 〉 0.05）;the level in MDS group （195.95± 117.39） was higher than that in control group（P 〈 0.05）, high risk group（229.25± 139.68） was higher than low risk group（171.73 ± 98.07）,but the difference was not significant（P 〉 0.05）. AML group （608.44 ± 342.58） was higher than that of MDS group （P 〈 0.01）. Conclusion：The number of tryptase-positive mast cells in the bone marrow of MDS patients was increased and may play a role in the pathogenesis and development of the disease.

关 键 词：骨髓增生异常综合征 急性髓性白血病 肥大细胞类胰蛋白酶 免疫组化 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]