顶空GC-MS法测定去甲斑蝥素中的有机溶剂残留  

Determination of Residual Organic Solvents in Norcantharidin by Headspace GC-MS

作　　者：冯润良[1] 宋智梅[1,2] 

机构地区：[1]济南大学化学化工学院,济南250022 [2]山东大学药学院

出　　处：《中国药师》2009年第6期716-718,共3页China Pharmacist

基　　金：济南大学博士启动基金(编号B0511)

摘　　要：目的:建立去甲斑蝥素中有机溶剂残留量的测定方法。方法:采用顶空毛细管气相色谱法,HP-5MS柱(30m×0.25 mm×0.25μm),火焰离子化检测器(FID),以水为溶剂,进行气相色谱分析,并以电子轰击(EI)为离子源,进行质谱(MS)分析。结果:证实残留溶媒为丙酮。结论:本方法简单、准确、灵敏,重复性好,适用于去甲斑蝥素中有机溶剂残留量的测定。Objective： To establish a method for determination of residual organic solvents in Norcantharidin. Method： The samples were dissolved in water and were injected into HP-5 MS capillary column （30m × 0.25ram× 0.25μm） by headspace sampler and analysed with FID detector. The structure of the residual solvent was determined by MS spectrum with EI as ion source. Result： The residual organic solvent was acetone. Conclusion： The method is simple, accurate and sensitive with good reproducibility. It can be used for the detemination of the residual solvents in Norcantharidin.

关 键 词：去甲斑蝥素 顶空气相色谱-质谱法 有机溶剂 残留量 

分 类 号：TQ460.72[医药卫生—药物分析学]