胚胎发育信号通路Sonic hedgehog在胰腺癌组织中的表达及意义  

Expression and significance of sonic hedgehog in human pancreatic cancer

作　　者：胡伟国[1] 熊炯邝[1] 王春友[1] 刘涛[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院胰腺外科中心,武汉430022

出　　处：《中华肝胆外科杂志》2009年第5期368-370,共3页Chinese Journal of Hepatobiliary Surgery

基　　金：本课题受国家自然科学基金资助（项目编号：30571817）

摘　　要：目的研究胚胎发育信号通路Sonic hedgehog（SHH）在胰腺癌组织中的表达及其临床意义。方法逆转录聚合酶链反应（RT—PCR）技术和免疫组化方法检测胰腺癌组织及癌旁组织中SHH mRNA和蛋白的表达情况。结果78．9％（30／38）胰腺癌组织中检测到SHH mRNA；18．4％（7／38）癌旁正常组织SHH mRNA阳性。免疫组化结果显示：84．2％（32／38）胰腺癌组织SHH蛋白呈阳性表达；癌旁胰腺组织SHH阳性率为21．1％（8／38）。两组差异有显著意义（P〈0．01）。SHH蛋白表达与年龄、肿瘤大小、TNM分期、淋巴结转移状况、组织学类型和肿瘤部位均无关（P〉0．05）。结论SHH信号分子在胰腺癌组织中表达增高，SHH信号途径可能在胰腺癌发生发展过程中起重要作用。Objective To investigate the expression of sonic hedgehog （SHH） and its clinical significance in human pancreatic cancer （hPC）. Methods RT-PCR and immunohistochemistry were used to determine the mRNA and protein expression of SHH in hPC tissue and normal tissues adjacent to the cancer. Results The SHH mRNA expression was detected in 78.9% （30/38） of the samples of pancreatic cancer, while in only 18.4% （7/38） of those of normal tissues adjacent to the cancer. Immunohistochemical analysis showed that the SHH protein expression rate was 84.2% （32/38） in pancreatic cancer tissue and 21.1% （8/38） in normal tissues. There was significant difference between them （P〈0.01）. Overexpression of SHH protein in pancreatic cancer had no correlation to age, tumor size, lymph node metastasis, TNM stage and pathological type （P〉0.05）. Conelusion The expression level of SHH signal molecule is increased in hPC. The overexpression of SHH signaling pathway might play an important role in carcinogenesis and development of pancreatic cancer.

关 键 词：胰腺肿瘤 Sonic HEDGEHOG 

分 类 号：R686[医药卫生—骨科学]