Ras相关区域家族1A半乳糖凝集素3和甲状腺过氧化物酶mRNA在甲状腺乳头状癌组织中的表达及临床意义  

Expressions of RASSF1A, Galectin-3 and TPO mRNA in papillary thyroid carcinoma and their clinical significance

作　　者：徐美荣[1] 陈云[3] 周绍荣[1] 池鸣鸣[2] 陈森林[4] 刘磊玉 

机构地区：[1]南通大学附属医院普外科,226001 [2]南通大学附属医院病理科,226001 [3]中国人民解放军第四一一医院病理科 [4]中国人民解放军第四一一医院普外科 [5]上海宝山区中心医院病理科

出　　处：《中华肿瘤杂志》2009年第5期356-360,共5页Chinese Journal of Oncology

基　　金：2006年上海市虹口区卫生局重点课题（0602-09）

摘　　要：目的探讨Ras相关区域家族1A（RASSF1A）、半乳糖凝集素3（Galectin-3）和甲状腺过氧化物酶（TPO）mRNA在甲状腺乳头状癌组织及其他滤泡源性良性病变组织中的表达情况和临床意义。方法收集73例甲状腺肿块手术标本，其中甲状腺乳头状癌23例，结节性甲状腺肿16例，甲状腺腺瘤29例，桥本氏病5例。采用逆转录聚合酶链反应（RT—PCR），检测73例甲状腺组织标本中RASSF1A、Galeetin-3和TPOmRNA的表达情况。结果RASSF1A、Galectin-3和TPO mRNA在23例甲状腺乳头状癌组织中的阳性表达率分别为26．1％、82．6％和21．7％，均与其他滤泡源性良性病变中的表达率差异有统计学意义（均P〈0．05）；RASSF1A、Galectin-3和TPO mRNA在各滤泡源性良性病变中的表达率差异均无统计学意义（均P〉0．05）。在甲状腺滤泡源性良恶性病变中，RASSF1AmRNA与Galeetin-3mRNA的表达呈负相关（Pearson点相关系数：-0．4090，χ^2=12．2088，P=0．000）；RASSF1AmRNA与TPOmRNA的表达呈正相关（Pearson点相关系数=0．2763，χ^2=4．8217，P=0．028）。结论RASSF1A和TPOmRNA在甲状腺乳头状癌组织中的表达普遍缺失，而Galectin-3mRNA表达率较高。RASSF1A、Galectin。3和TPOmRNA与甲状腺乳头状癌的发生和发展密切相关，可作为诊断和鉴别诊断甲状腺乳头状癌的标志物，且联合检测的结果更可靠。Objective To investigate the mRNA expressions of RASSF1A, Galeetin-3 and TPO in papillary thyroid carcinoma and some other thyroid benign lesions, and evaluate their diagnostic significance. Methods Reverse transcription polymerase chain reaction （RT-PCR） was used to detect the mRNA expression of RASSF1 A, galectin-3 and TPO in the samples from 73 cases, including 23 cases with papillary thyroid cancer, 16 with nodular goiter, 29 with thyroid adenoma and 5 with Hashimoto＇s disease. Results A statistically significant difference in the mRNA expression of RASSF1 A, Galectin-3 and TPO was observed between papillary thyroid carcinoma and follicular benign lesions （ P 〈 0.05 ）. However, there was no significant difference among various kinds of benign lesions （ P 〉 0. 05 ）. A negative correlation of the expression of RASSF1A and Galeetin-3 mRNA was found between thyroid benign lesions and malignant ones （P= 0. 000）. While the mRNA expression of RASSF1A and TPO was positively correlated between benign and malignant lesions （P = 0. 028）. Conclusion Loss of expression of RASSF1A and TPO mRNA but high expression of Galectin-3 mRNA in papillary thyroid carcinoma are common. Therefore, the products of these three genes may be closely related to the development of thyroid papillary carcinoma, and may be used as useful markers in differential diagnosis of papillary thyroid carcinoma from the benign lesions. The results are more reliable if this detection method is used in combination with other techniques.

关 键 词：甲状腺肿瘤 逆转录聚合酶链反应 RAS相关区域家族1A 半乳糖凝集素3 甲状腺过氧化物酶 

分 类 号：R686[医药卫生—骨科学]