应用MTT比色法评价不同牛血清促细胞生长作用  

The Application of the MTT to Estimate the Effect on Cell Growth by Using the Different Bovine Serum

作　　者：张宝红[1] 钟恒禄[1] 卢惠芳[1] 张为宇[1] 林莹[2] 韩忠燕[1] 黄海愉[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530005 [2]广西大学食品轻工学院,南宁530004

出　　处：《中国畜牧兽医》2009年第5期84-86,共3页China Animal Husbandry & Veterinary Medicine

基　　金：南宁市市校科技合作项目(200801031B);广西大学博士启动基金项目(x081006)

摘　　要：细胞培养过程中血清的选择对细胞的促生长增殖具有重要作用。本试验分别以商品新生牛血清、自制新生牛血清、自制成年牛血清培养成纤维细胞、骨髓瘤细胞、杂交瘤细胞,通过MTT比色法来判断细胞的增殖能力,进而对血清质量作出评价。结果表明,自制新生牛血清具有良好的促细胞生长作用(相对生长率>0.96),并且3次试验结果比较差异不显著(P>0.05),具有较好的重复性;而成年牛血清和无血清空白对照组促细胞作用不明显。因此应用MTT比色法能够评价血清质量。The preference of serum is very significant during the cell culture. The experiment used the commercial serum, self-made newborn bovine serum and self-made adult bovine serum to culture mechanocyte, myeloma cell and hybridoma cell and then applied the MTT to verify the cell growth better or worse, so that we can evaluate the quality of the serum. The resuits demonstrated that self-made newborn bovine serum was the best for cell growth （the relative growth rate of every above ceils exceed 0.96）, the three experimental data showed the result was not different （P〉0.05）, so the self-made newborn bovine serum was more stable. However the self-made adult bovine serum and no serum comparison had not the significant effect to promote the cell growth. So the MTT could estimate the quality of the serum.

关 键 词：新生牛血清 MTT比色法 相对生长率 血清质量 

分 类 号：S813.11[农业科学—畜牧学]