巢湖鸭生长素基因和垂体特异性转录因子1基因的PCR-SSCP检测  

PCR-SSCP Detection of Ghrelin and Pit-1 Gene in Chaohu Duck

作　　者：李俊营[1] 詹凯[1] 许月英[1] 王德圣 赵瑞宏[1] 唐中节 

机构地区：[1]安徽省农业科学院畜牧兽医研究所,合肥230031 [2]江苏省海安县海安镇畜牧兽医站,海安226600 [3]安徽省庐江县巢湖鸭保种场,庐江231490

出　　处：《中国畜牧兽医》2009年第5期107-111,共5页China Animal Husbandry & Veterinary Medicine

基　　金：安徽省“十一五”科技攻关计划资助项目(07010302132);国家科技支撑计划资助项目(2008BADC1B03)

摘　　要：以生长素基因Ghrelin和垂体特异性转录因子1Pit-1基因为候选基因,采用PCR-SSCP和DNA测序技术检测2个候选基因在巢湖鸭群体中的单核苷酸多态性(SNPs)。结果表明,Ghrelin基因exon 3第54 bp位置有G→A碱基的点突变,在巢湖鸭群体中检测到AA、AB、BB 3种基因型,A等位基因频率为0.49,B等位基因频率为0.51;在exon 5第149位和166位发生了C→A和G→T的突变,检测到MM、MN、NN 3种基因型,M等位基因频率为0.19,N等位基因频率为0.81。Pit-1基因的2对引物的扩增片段均未检测到多态性,说明所检测的Pit-1基因外显子3和4序列比较保守。Ghrelin gene and pituitary specific transcription factor 1 （Pit-1） gene were studied as candidate genes on the Cha- ohu duck in this study. Polymorphisms of Ghrelin and Pit-1 gene had been analyzed by PCR-SSCP and DNA sequencing method. The results showed that three SNPs were identified at Ghrelin gene, one SNP （G54A） located at the third exon, another two in the fifth exon were transitions of C to A （149） and G to T （166）. The frequency of A/B alleles for Ghrelin gene in the population was 0.49 and 0.51, and that of M/N alleles was 0.19 and 0.81. No polymorphisms were detected in the amplified regions of two pairs of primers in Chaohu duck population tested, which may indicate that the sequences of exons 3 and 4 of Pit- 1 gene were rather conserved.

关 键 词：巢湖鸭 生长素基因 垂体特异性转录因子1 SNPS 

分 类 号：S834.2[农业科学—畜牧学]