不同浓度的IGF-1对山羊毛囊干细胞体外培养的影响  

Effect of Different Concentrations of IGF-1 on the Culture of Goat Hair Follicle Stem Cells

作　　者：史明艳[1,2] 高雪[3] 杨学义[1] 窦忠英[2] 

机构地区：[1]洛阳师范学院生命科学系,洛阳471022 [2]西北农林科技大学国家干细胞工程技术研究中心陕西分中心,杨凌712100 [3]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国畜牧兽医》2009年第5期127-129,共3页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(30200137);国家“863”计划(2002AA216161);教育部重大科技专项(03160)

摘　　要：采用2.4 U/mL Dispase酶消化和机械切割法分离毛囊隆突部,经胰酶(0.5 mg/mL胰酶+0.2 mg/mL EDTA)消化,从山羊耳部皮肤分离得到毛囊干细胞,并检测了不同浓度的IGF-1对山羊毛囊干细胞体外培养的作用。结果表明,IGF-1对毛囊干细胞体外培养作用很明显,并且最适IGF-1浓度是10 ng/mL。Hair follicles were isolated from goat ear skin by 2.4 U/mL Dispase digestion and the bulges were separated with special fine needle and digested with 0.5 mg/mL trypsin plus 0.2 mg/mL EDTA. The results showed that cells were cultured in different concentrations of IGF-1, IGF-1 had a good effect on the goat hair follicle stem cells in vitro and the 10 ng/mL was best concentration.

关 键 词：毛囊干细胞 克隆形成率 IGF-1 体外培养 

分 类 号：Q813.11[生物学—生物工程]