猪繁殖与呼吸综合征病毒实验室检测技术研究进展  

Researching Progress of Laboratory Diagnostic Technique in Porcine Reproductive and Respiratory Syndrome Virus

作　　者：仝利剑[1,2,3] 张社民 高英杰[1] 赵建增 慕泽鑫[1] 罗胜军[4] 温涛[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]山西省芮城县家畜家禽疫病防治站,芮城044600 [3]大北农动物医学研究中心,北京100080 [4]广东省农业科学院兽医研究所,广州510640

出　　处：《中国畜牧兽医》2009年第5期138-141,共4页China Animal Husbandry & Veterinary Medicine

基　　金：山西省科技攻关计划项目(20080311036-2)

摘　　要：猪繁殖与呼吸综合征(PRRS)的临床症状表现为母猪繁殖性能下降、不孕、流产等。感染猪繁殖与呼吸综合征病毒(PRRSV)后病理及组织学变化往往不太典型,而且易与其它病原感染所引起的病理变化相混淆,给该病的诊断带来一定困难,因此,对于PRRSV的确诊有赖于实验室诊断,不同的诊断方法敏感性、特异性及成本方面存在差异,而现有的血清学方法无法区分疫苗抗体和野毒抗体。作者就PRRSV抗原或抗体的检测技术(如病毒分离、RT-PCR、免疫过氧化物酶技术、酶联免疫吸附试验等)的研究现状作一简单的概述。The syndrome of porcine reproductive and respiratory syndrome （PRRS） is characterized by respiratory disease leading to increased mortality in piglets and decreased performance infertility or late-term abortions in sows. The change of pathology and microanatomy was not often typical in infected pig, it was easy to confused with other pathological changes caused by other etiological agents, it is difficult to diagnose. The definite diagnosis was depended on laboratory diagnosis to increase the level of diagnosis and prevention. The sensitivity, specificity and cost are different in different laboratory diagnostic assays, serology test can＇t differentiate antibodies caused by field virus or vaccine. The research about methods of viral RNA detection, viral antigens, and antibodies in this article were discussed.

关 键 词：猪繁殖与呼吸综合征病毒 实验室检测技术 

分 类 号：S852.659.6[农业科学—基础兽医学]