北京地区G1-G4型人轮状病毒地方株VP7编码基因的序列分析  

SEQUENCE ANALYSIS OF THE GENES ENCODING VP7 PROTEINS FROM FOUR HUMAN ROTAVIRUS FIELD STRAINS REPRESENTING G1-G4 SEROTYPES

作　　者：李国华[1] 钱渊[1] 熊朝晖[1] 靖宇[1] 

机构地区：[1]首都儿科研究所北京感染与免疫中心实验室

出　　处：《病毒学报》1998年第2期126-132,共7页Chinese Journal of Virology

摘　　要：本文报告了北京地区流行的４个轮状病毒地方株（Ｇ１－Ｇ４）ＶＰ７编码基因的核苷酸序列。４个地方株的该基因核苷酸全长均为１０６２ｂｐ，读码框架和已往的研究一致。地方株和相同血清型的标准株之间ＶＰ７氨基酸序列具有高度同源性（９２％－９４％），而不同血清型间则变异较大（６９％－８０％）。不同血清型间氨基酸序列的变异主要存在于高变区内，高变区以外的区域在不同血清型轮状病毒间保守。这一序列分析结果进一步从分子水平分析了地方流行毒株的血清型别。Genes coding for the major neutralizing protein(VP7) from four human rotavirus field strains representing serotype 1、2、3 and 4 were cloned and sequenced.These field strains were identified from stool specimens from children with diarrhea in Beijing area.All the VP7 genes from these field strains are 1 062bp long and contain two open reading frames which consist with that reported in the literature.The deduced amino acid identity is high among the VP7 genes of the field strains and the reference strains of same serotypes(92%-94%),whereas among different serotypes,sequences are highly divergent(69%-80%).The divergence of amino acid sequences is mainly within the nine divergent regions.

关 键 词：轮状病毒 VP7 序列分析 

分 类 号：R373.2[医药卫生—病原生物学]