Epstein-Barr病毒诱导永生化人上皮细胞恶性转化  

EPSTEIN-BARR VIRUS INDUCED MALIGNANT TRANSFORMATION OF IMMORTALIZED HUMAN EPITHELIAL CELLS

作　　者：李宝民[1,2] 纪志武[1,2] 刘振声[1,2] 曾毅[1,2] 

机构地区：[1]中国预防医学科学院病毒学研究所 [2]西安第四军医大学西京医院

出　　处：《病毒学报》1998年第2期133-138,共6页Chinese Journal of Virology

摘　　要：为了使ＥＢ病毒能直接感染人上皮细胞，将ｐＳＧ－ＣＲ２－Ｈｙｇ载体转入永生化人上皮细胞（２９３细胞）中。通过间接免疫荧光法测定发现，转化的细胞表达ＥＢ病毒受体。用ＥＢ病毒感染ＣＲ２－２９３细胞后，２３％的细胞可表达ＥＢ病毒抗原。用ＴＰＡ作用于这些细胞后，表达病毒抗原的细胞数增加。用ＰＣＲ法从ＥＢ病毒感染细胞ＤＮＡ中扩增出ＥＢ病毒ＤＮＡＷ片段。在ＴＰＡ持续作用下，ＥＢ病毒感染细胞的形态特征发生改变。把ＥＢ病毒感染细胞接种在裸鼠皮下，每周注射ＴＰＡ，可诱导细胞在裸鼠体内形成肿瘤。经组织病理检查确诊，肿瘤为低分化上皮细胞癌。杂交试验证明，肿瘤组织细胞中有ＥＢ病毒ＥＢＥＲｓ存在。上述结果表明，ＥＢ病毒在ＴＰＡ协同作用下，可诱导永生化上皮细胞恶性转化。In order to investigate the role of EB virus in the transformation of human epithelial cells,pSG-CR2-Hyg vector was transfected into immortalized human epithelial cells(293 cells)to express. EBV receptors. EBV was used to infect these CR2-293 cells, in which EBV could replicate and express EBV antigens.EBV W fragments were amplified in EBV-infected CR2-293 cells by PCR,EBV-infected epithelial cells grew in piles with multiple cellular layers.When these epithelial cells were transplanted subcutaneously into nude mice and treated with TPA,poorly differentiated carcinomas were induced.Hybridization test indicated that EBERs of EBV were present in tumor tissues.

关 键 词：鼻咽癌 EB病毒 上皮细胞 癌变 

分 类 号：R373.1[医药卫生—病原生物学] R739.630.2[医药卫生—基础医学]