培养基除菌过滤在百日咳菌苗生产中的应用  

Application of Media Filter Sterilization to the Production of Pertussis Vaccine

作　　者：刘保奎[1] 刘文达[1] 周第 王久芳 陈春兰 白荣英 

机构地区：[1]卫生部北京生物制品研究所

出　　处：《微生物学免疫学进展》1998年第2期17-20,共4页Progress In Microbiology and Immunology

摘　　要：经一系列试验证实百日咳菌大罐培养浓度的下降与培养基中不耐热营养因子不足有关，将培养基的高压灭菌方式改为除菌过滤，保留热不稳定营养因子，可以提高百日咳菌大罐培养浓度；优化酸沉淀条件后，提高了菌苗回收率，从而节省了大量培养基，缩短了生产时间，获得了良好的经济效益。The decrease of fermenter culture concentration of pertussis bacteria is related to the insufficiency of heat labile growth factor in media confirmed by a series of experiments.The bacteria concentration in fermenter culture was increased by means of sterilizing the media with filtration which retained the heat labile growth factor instead of autoclave,and the rate of culture recovery was improved by the optimization of acid precipitation conditions.Therefore,a good economic result is gained by saving both the production time and the amount of media used in the production of pertussis vaccine.

关 键 词：百日咳菌苗 大罐培养 高压灭菌 除菌过滤 

分 类 号：R392－33[医药卫生—免疫学] R516.601[医药卫生—基础医学]