紫羊茅抗铜和敏感品种在发育早期对铜离子反应的生理差异  

PHYSIOLOGICAL DIFFERENCES OF FESTUCA RUBRA COPPERTOLERANT MERLIN AND SENSITIVE S59 TO COPPERTREATMENT AT THEIR EARLY DEVELOPMENTAL STAGES

作　　者：黄玉山 邱国华[2] 

机构地区：[1]香港九龙城市大学生物及化学系 [2]中山大学生物工程研究中心,广州510275

出　　处：《应用与环境生物学报》1998年第2期126-131,共6页Chinese Journal of Applied and Environmental Biology

摘　　要：紫羊茅（Festucarubra）抗铜品种Merlin和敏感品种559种子在水中吸胀5d后长出的幼苗在一般培养液中分别培养了0、2、4、6、8和10d（分别称为D0、D2、D4、D6、D8和D10）后再置于ρ（Cu2＋）＝5mg／L的相同培养液中，其中约一半培养至d20，其余至d27．结果与同龄的幼苗相比，MerlinD6在27d和20d的根中均有最高的干物质含量和最大的叶绿素浓度；在27d苗的根中铜积累了最少；而MerlinD6的苗在各个抗性处理组中最高在从20d至27d的培养期间，MerlinD6地上部分的干物质增加最多，而根中则减少最多，叶绿素含量也减少最多，在根中积累的铜也最少；可是在敏感的S59中则没有这种特殊现象，这些现象暗示，即使是在抗性品种的Merlin中，在其不同的生长时期经铜处理，也表现出不同的抗性，并可能有一个抗性最大的生长期。Festuca rubra copper tolerant Merlin and sensitive 559 seedings that socked in deionized water for5 d were divided into seven groups, six of which were cultured respeCtively in normal media for 0, 2, 4, 6, 8and 10 d, and correspondingly named as D1, D2, D4, D6, D8 and D10 prior to their exposure to copper at themass concentration (ρ ) of 5 mg. L- 1 in the same media, and the rest one was grown in normal media throughout the culture. About half of each was harvested on 20 d and the others on 27 d. Among these seedlings, Merlin D6 had the highest dry material contents in shoots on 27 d and in roots on 20 d maximum chlorophyll contents on both d 20 and 27 seedlings, minimal copper accumulation in the rootS of seedlings on 27 d, and thetallest shoots in Merlin seedlings on 20 d. FOr seedlings on 20 d, Merlin D6 showed a maximum increase inshoots and maximum decrease in roots of dry mass production, maximum reduction of chlorophyll content andminimal quantities of copper accumulation in roots after the further culture for a week. These results indicatedthat even in the copper tOlerant Merlin, different tolerance to metal might express and a maximal tolerant pointmight exist when Merlin was treated by copper at the different developmental stages.

关 键 词：紫羊茅 重金属抗性 铜 生理反应 

分 类 号：Q945.14[生物学—植物学] Q949.714.2