雪松聚球藻对重金属镉的抗性和解毒作用  

TOLERANCE TO HEAVY METAL Cd AND DETOXIFICATIONOF SYNECHOCOCCUS CEDRORUM

作　　者：戴玲芬[1] 高宏[1] 夏建荣[1] 

机构地区：[1]中国科学院本生生物研究所,武汉430072

出　　处：《应用与环境生物学报》1998年第2期192-195,共4页Chinese Journal of Applied and Environmental Biology

基　　金：中国科学院水生生物研究所所长基金

摘　　要：培养液中不同浓度（c）的重金属镉（Cd）对雪松聚藻（Synechococcuscedrorum）有相当的致毒作用，当c（Cd）＞2μmol／L时，藻体的生长和光合作用均明显受到抑制，c＞10μmol／L时，藻细胞出现解体，光合放氧量几近0,然而，当按梯度逐级提高培养基中c（Cd）时，雪松聚球藻则相应产生对有毒金属Cd的抗性，在即使c（Cd）达20μmol／L的培养基中亦生长良好．为探讨该藻对重金属致毒的抗性产生机理及其解毒作用，对藻体蛋白和胞内Cd进行了分离检测，结果表明：Cd在进入藻细胞后，与胞内多肽结合形成一种大分子量的色素蛋白复合体，和一种低分子量的金属结合蛋白，特别是后一种蛋白在藻细胞中的含量明显受外界Cd的诱导，并起解毒作用．The growth rate, photosynthesis and pigmentation of the blue-green alga Synechococcus cedrorum were markedly affected by adding≥ 2 μmol/L Cd in medium. The cell breakage and more than 80% loss ofphotosynthetic oxygen evolution appeared when the alga were tranferred from normal growth medium to one with10 μmol/L Cd. With gradual elevation of the metal in the medium, S. cedrorum could tolerate Cd level up to20 μmol/L without obivous changes in growth rate, oxygen evolution and pigments ratio . It seems that a strongtolerance of the alga ic Cd was induced during subsequent culture in the medium with progressive addition of themetal. The analyses and determination of cellular Cd and isolated protein from the adapted algal cells indicatedthat after permeating into the cells, the metal could be bound by a low molecullar peptide and high molecullarpigment protein complex in the algal cells. Both of the proteins, especially the former made the alga detoxifyfrom heavy metal Cd.

关 键 词：藻类 雪松聚球藻 Cd 金属结合蛋白 解毒 

分 类 号：Q949.22[生物学—植物学] X703[环境科学与工程—环境工程]