Fingerprinting Analysis of Rhizoma Chuanxiong of Commercial Types using ~1H Nuclear Magnetic Resonance Spectroscopy and High Performance Liquid Chromatography Method  

作　　者：Hai-Lin Qin An-Jun Deng Guan-Hua Du Peng Wang Jin-Lan Zhang Zhi-Hong Li 

机构地区：[1]Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, Key Laboratory of Bioactive Substances and Resources of Chinese Herbal Medicine, Ministry of Education, Beijing 100050, China

出　　处：《Journal of Integrative Plant Biology》2009年第6期537-544,共8页植物学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China (30630073);the Science Foundation of the State Administration of Traditional ChineseMedicine, China (02-03ZP09)

摘　　要：The ^1 H nuclear magnetic resonance （^1 H NMR） fingerprints of fractionated non-polar extracts （control substance for a plant drug （CSPD） A） from Rhizoma chuanxiong, the rhizomes of Ligusticum chuanxiong Hort., of seven specimens from different sources were measured on Fourier Transform （FT）-NMR spectrometer and assigned by comparing them with the ^1 H NMR spectra of the isolated pure compounds. The ^1 H NMR fingerprints showed exclusively characteristic resonance signals of the major special constituents of the plant. Although the differences in the relative intensity of the ^1H NMR signals due to a discrepancy in the ratio of the major constituents among these samples could be confirmed by high performance liquid chromatography analysis, the general features of the ^1H NMR fingerprint established for an authentic sample of the rhizomes of L. chuanxiong e. hibited exclusive data from those special compounds and can be used for authenticating L. Chuanxiong species.The ^1 H nuclear magnetic resonance （^1 H NMR） fingerprints of fractionated non-polar extracts （control substance for a plant drug （CSPD） A） from Rhizoma chuanxiong, the rhizomes of Ligusticum chuanxiong Hort., of seven specimens from different sources were measured on Fourier Transform （FT）-NMR spectrometer and assigned by comparing them with the ^1 H NMR spectra of the isolated pure compounds. The ^1 H NMR fingerprints showed exclusively characteristic resonance signals of the major special constituents of the plant. Although the differences in the relative intensity of the ^1H NMR signals due to a discrepancy in the ratio of the major constituents among these samples could be confirmed by high performance liquid chromatography analysis, the general features of the ^1H NMR fingerprint established for an authentic sample of the rhizomes of L. chuanxiong e. hibited exclusive data from those special compounds and can be used for authenticating L. Chuanxiong species.

关 键 词：control substance for a plant drug A ^1 H nuclear magnetic resonance fingerprint high performance liquid chromatography Ligusticumchuanxiong phenylpropenoid phthalides. 

分 类 号：O657.2[理学—分析化学] TS513[理学—化学]