Development of a Low-cost Polymerase Chain Reaction-based Method for Studying Differentially Expressed Genes in Developing Rice Leaves  

作　　者：Yin-Wan Wendy Fung Hoi Yee Chow Tik Wan Law Biao Dong Hoi Shan Kwan 

机构地区：[1]Faculty of Science, The Chinese University of Hong Kong, Shatin, Hong Kong SAP, China [2]Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China

出　　处：《Journal of Integrative Plant Biology》2009年第6期614-621,共8页植物学报（英文版）

基　　金：Supported by the Area of Excellence Grant on Plant and Fungal Biotechnologyfrom the University Grants Committee of the Hong Kong SAR Government

摘　　要：Gene expression studies are important for revealing gene functions putatively involved in biological processes. We were interested in identifying differentially expressed genes during leaf development in rice. We combined the RNA arbitrarily primed-polymerase chain reaction （RAP.PCR） and dot blot hybridization methods to screen a rice leaf primordium cDNA library. Three developmental stages during vegetative growth were examined. The cDNA clones showing different hybridization patterns were further analyzed and verified. Here we demonstrate that the combination of RAP-PCR and dot blot hybridization could provide an efficient and relatively low-cost cDNA library screening approach to discover genes not previously known to be associated with leaf development in rice, We believe that the findings described here will help to elucidate the molecular mechanism（s） underlying the developmental processes of rice leafGene expression studies are important for revealing gene functions putatively involved in biological processes. We were interested in identifying differentially expressed genes during leaf development in rice. We combined the RNA arbitrarily primed-polymerase chain reaction （RAP.PCR） and dot blot hybridization methods to screen a rice leaf primordium cDNA library. Three developmental stages during vegetative growth were examined. The cDNA clones showing different hybridization patterns were further analyzed and verified. Here we demonstrate that the combination of RAP-PCR and dot blot hybridization could provide an efficient and relatively low-cost cDNA library screening approach to discover genes not previously known to be associated with leaf development in rice, We believe that the findings described here will help to elucidate the molecular mechanism（s） underlying the developmental processes of rice leaf

关 键 词：cDNA clones DEVELOPMENT dot blot hybridization PRIMORDIUM RNA arbitrarily primed-polymerase chain reaction. 

分 类 号：Q943.2[生物学—植物学] S511[农业科学—作物学]