雄性无隐睾尾部悬吊大鼠及其心肌功能  

Tail-suspended Rats with Inguinal Canal Ligation and Their Myocardial Function.

作　　者：余志斌[1] 谢小萍[1] 暴军香[1] 马进[1] 张立藩[1] 

机构地区：[1]第四军医大学航空生理学教研室

出　　处：《航天医学与医学工程》1998年第3期173-176,共4页Space Medicine & Medical Engineering

基　　金：国家自然科学基金

摘　　要：为防止大鼠尾部悬吊期间，睾丸滑入腹腔造成隐睾症，建立了无隐睾雄性尾部悬吊大鼠模型。结果表明：采用腹股沟管环缩术，可有效地防止睾丸滑入腹腔，且不影响睾丸的形态与功能。尾部悬吊４周大鼠睾丸重量（对照组：１．７０±０．１９ｇ，悬吊组：０．６０±０．１６ｇ）与血清睾酮含量（对照组：９９．５±３２．７ｎｇ／ｄｌ，悬吊组：２３．０±１３．２ｎｇ／ｄｌ）明显降低（Ｐ＜０．０１），无隐睾悬吊组（ＳＴ）的睾丸重量（１．２１±０．３０ｇ）与血清睾酮水平（４０．７±３２．６ｎｇ／ｄｌ）亦下降，但其均值介于对照组与悬吊组之间。光镜观察发现悬吊组睾丸组织的曲精细管均明显萎缩，精原细胞退化与坏死，数量明显减少，曲精细管内精子数量减少。但ＳＴ组萎缩的程度较轻，且未见精原细胞坏死现象。悬吊组与ＳＴ组大鼠心肌等长收缩的静息张力无改变，发展张力均明显降低，血清睾酮水平与心肌发展张力间无相关关系。To examine the effect of testosterone level on myocardial contractility and to prevent the testis from sliding into abdominal cavity when subjected to tail-suspension,a new tail-suspended model of rat with its inguinal canal ligated was made.Rats suspended for 4 w without inguinal canal ligation (SUS) exhibited significant reduction in weight of testis (CON 1.70±0.19 g;SUS 0.60±0.16 g) and in serum testosterone level (CON 99.5± 32.7 ng/dl;SUS 23.0±13.2 ng/dl)(P<0.01).Unexpectedly,4 w tail-suspension of rats with inguinal canal ligation (ST) also showed significant changes in testis weight ( 1.21±0.30 g) and serum testosterone level (40.7±32.6 ng/dl).A significant decrease of the developed tension of the papillary muscle of the left cardiac ventricle was observed in SUS and ST group as compared with controls (CON:3.93±0.42 g/mm 2, SUS: 2.54±0.85 g/mm 2, ST: 2.60±0.73 g/mm 2).There was no relationship between the developed tension and serum testosterone level.It suggests that the decrease of blood supply of the testis may result in an atrophy of the testis in ST group.

关 键 词：睾丸 睾酮 心肌收缩 大鼠 悬吊 模拟失重 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]