复方氯化铵甘草口服溶液中麻醉成分的质控研究  

Study on Quality Control of the Components of Anesthesia in Compound Ammonium Glycyrrhiza Oral Solution

作　　者：张钢平[1] 

机构地区：[1]河北省邯郸市药品检验所,邯郸056001

出　　处：《中国药事》2009年第5期425-427,共3页Chinese Pharmaceutical Affairs

基　　金：河北省科学技术研究与发展指导计划项目(编号07276476)

摘　　要：目的建立制剂中麻醉成分薄层色谱的有效分离方法;建立HPLC法测定制剂中吗啡的含量。方法选用吗啡、磷酸可待因、盐酸罂粟碱为对照品;采用AgilentZORBAXSB-C8色谱柱;流动相:0.05mol.L-1磷酸二氢钾溶液-0.0025mol.L-1庚烷磺酸钠溶液-乙腈(18∶18∶5),检测波长:220nm。结果定性三种麻醉成分,并可有效分离,吗啡在0.5225～20.90μg.mL-1范围内呈良好线形关系,r=1.0000。结论本法分离度好,快速,简便。Objective To establish the effective separation method of narcotic ingredients TLC in pharmaceuticals; HPLC method was described to determine morphine. Methods Select morphine, codeine phosphate and papaverin hydrochloride as reference substances. A Agilent ZORBAX SB-C8 column was used. The mobile phase was 0.05mol·L^-1 KH2PO4-0. 0025mol·L^-1 Sodium heptane-acetonitrile （18 ： 18 ： 5）. The content was detected at a wavelength of 220nm. Results To characterize the three components of anesthesia and have an effective separation of them. The linear range was 0. 5225-20. 90μg·mL^-1 with r=1. 0000. Conclusions The method is simple, accurate and suitable.

关 键 词：三种麻醉成分 薄层色谱 HPLC 

分 类 号：R284.1[医药卫生—中药学] R927.2[医药卫生—中医学]