GS0202菌产人参皂苷糖苷酶的酶性质研究  

Properties of Panaxsaponin-glucosidase from Arthrobacter chlorophenolicus GS0202

作　　者：张妍[1] 金凤燮[1] 鱼红闪[1] 

机构地区：[1]大连工业大学生物与食品工程学院,辽宁大连116034

出　　处：《食品与发酵工业》2009年第4期16-19,共4页Food and Fermentation Industries

基　　金：国家自然科学基金资助项目(20476017);辽宁省高等学校优秀人才支持计划资助项目(RC-04-05);辽宁省科学技术基金项目(20042132)

摘　　要：对Arthrobacter chlorophenolicus GS0202菌产人参皂苷糖苷酶进行分离纯化并对其性质进行了研究。结果表明,电泳纯人参皂苷糖苷酶的分子质量约为66ku,最适反应pH值为5.0,最适温度为40℃,在pH3.0～7.0,20～60℃酶活力相对稳定。K+、Na+、Zn2+对酶活性影响很小;Ca2+、Mg2+、Cu2+对酶活性有明显的抑制作用。Km值为4.392mmol/L,Vmax值为0.1275mmol/(h.L)。The paper was aimed to investigate panaxsaponin-glucosidase which was purified from the broth of microbion （Arthrobacter chlorophenolicus GS0202 ） and its properties. The results showed that the molecular weight of which was 66ku,the optimum pH and temperature were pH 5.0 and 40℃ respectively,the enzyme was stable in the pH range from 3.0 to 7.0 and the temperature ranges from 20℃to 60℃. K^＋ , Na^＋ and Zn^2＋ had little contribution to the enzyme; Ca^2＋ , Mg^2＋ and Cu^2＋ showed noticeable inhibiting attributes. The kinetic analysis revealed that the value of Km and VmAx was 4. 392mmol/L and 0. 1275mmol/（h · L） , respectively.

关 键 词：人参皂苷糖苷酶 分离纯化 酶活性 抑制作用 

分 类 号：R284.1[医药卫生—中药学]