HPLC法测定中药炮制辅料猪胆汁中胆红素的含量  

HPLC determination of bilirubin in pig bile of processing materials in traditional Chinese medicine

作　　者：李先端[1] 游修琪[1] 代婉莹[1] 顾雪竹[1] 毛淑杰[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《药物分析杂志》2009年第5期714-716,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：科技部社会公益研究专项(2005DIB1Z169)

摘　　要：目的:建立中药炮制辅料猪胆汁中胆红素含量测定方法。方法:采用Zorbax RX-C18色谱柱(4.6mm×250mm,5μm);以二甲基亚砜-乙腈-0.125mol·L-1醋酸铵水溶液(60∶60∶70)为流动相,其中醋酸铵溶液用冰醋酸调pH=5.3;流速1.0mL·min-1;检测波长452nm。结果:胆红素进样量在0.0113～0.1356μg范围内呈良好线性关系(r=0.9998);平均回收率(n=6)为98.13%,RSD为2.5%。结论:本方法准确、简便,重复性好,可为中药炮制辅料猪胆汁的质量控制提供科学依据。Objective ： To establish a method for determination of bilirubin in pig bile of processing materials in traditional Chinese medicine. Methods：Zorbax RX - C18 column （4.6 mm×250 mm, 5μm） was used. The mobile phase was dimethyl sulfoxide - acetonitrile - 0.125 mol·L^-1 ammonium acetate （ 60：60： 70, pH = 5.3 ）. The flow rate was 1. 0 mL·min^-1;The detective wavelength was 452 nm. Results ：The linear range of bilirubin was 0.0113 -0.1356 μg （r = 0. 9998 ） ;The average recovery was 98.13%, RSD was 2.5 %. Conclusion：This method is accu, rate, simple and reproducible, it can provide a scientific basis for quality control of pig bile of processing materials in traditional Chinese medicine.

关 键 词：中药炮制辅料 胆红素 含量测定 

分 类 号：R392-33[医药卫生—免疫学] R283.2[医药卫生—基础医学]