RP-HPLC法测定急性子中2种凤仙萜四醇苷类成分的含量  

RP-HPLC determination of two hosenkosides in seeds of Impatiens balsamina L.

作　　者：谭丽娜[1] 陆娟[1] 朱娜[1] 孟磊[1] 朱红微[1] 刘玉玮[2] 金永日[1] 李绪文[1] 

机构地区：[1]吉林大学化学学院,长春130021 [2]通化市卫生学校,通化134000

出　　处：《药物分析杂志》2009年第5期717-720,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:对急性子中2种主要凤仙萜四醇苷类成分凤仙萜四醇苷K、凤仙萜四醇苷A进行含量测定。方法:采用反相高效液相色谱-蒸发光散射检测法,使用Eclipse XDB-C18色谱柱(4.6mm×250mm,5μm),流动相为甲醇-水(梯度洗脱,0～22min时为50∶50,22～50min时为54∶46),流速0.8mL·min-1,柱温25℃。ELSD检测器条件:载气(空气)流速2.5L·min-1,漂移管温度89℃。结果:凤仙萜四醇苷K、A进样量在5～20μg范围内线性关系良好,相关系数(n=6)分别为0.9995和0.9992;平均回收率(n=6)均为98.0%,RSD均为1.3%。结论:本方法能够准确测定急性子中凤仙萜四醇苷K、A的含量,方法简便,结果可靠。Objective：To determine two hosenkosides（ hosenkoside K and hosenkoside A）in the seeds of Impatiens balsamina. Methods：Using RP- HPLC with ELSD method. Separation was carried out on Eclipse XDB -C18 column（4.6 mm×250 mm,5 μm）. A linear solvent gradient was used,with a mobile phase of methanol - water（0 - 22 rain ,50：50 ;22 -50 min ,54：46 ）. The flow rate was 0.8 mL·min^-1 at 25℃. The gas（air） flow rate was 2. 5 L·min^-1 and the temperature of drift tube for measurement was 89℃. Results： The linearity was in the range of 5 - 20μg for hosenkoside K and A, and the correlation coefficients （ n = 6） were 0. 9995 and 0. 9992 respectively; The average recoveries（ n = 6） for hosenkoside K and A were 98.0% （ RSD = 1.3% ） and 98.0% （ RSD = 1.3% ） respectively. Conclusion：This method is accurate, reliable and reproducible for determination of hosenkoside K and A in the seeds of Impatiens balsamina.

关 键 词：急性子 凤仙萜四醇苷 反相高效液相色谱 蒸发光散射检测器 含量测定 

分 类 号：R917[医药卫生—药物分析学]