HPLC法测定柴胡口服液中柴胡皂苷b_2的含量  

HPLC determination of saikosaponin b_2 in Radix Bupleuri oral liquids

作　　者：李军[1] 姜华[1] 石任兵[2] 

机构地区：[1]河南科技大学化工与制药学院,洛阳471003 [2]北京中医药大学中药学院,北京100102

出　　处：《药物分析杂志》2009年第5期743-745,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：河南科技大学博士科研启动基金资助(2007-09001242)

摘　　要：目的:建立柴胡口服液中柴胡皂苷b2的含量测定方法。方法:采用Hypersil C18色谱柱(250mm×4.6mm,5μm);流动相:乙腈-水,梯度洗脱(0～50min,30∶70→50∶50);流速:1.0mL·min-1;柱温:30℃;检测波长:252nm。结果:柴胡皂苷b2的进样量在0.03～0.30μg范围内,与其峰面积呈良好的线性关系(r=0.9998);平均加样回收率(n=6)为98.0%,RSD为0.9%。结论:所建立的方法灵敏、简便、准确,可用于该药中柴胡皂苷b2的含量测定。Objective ：To establish an HPLC method for the quantitative determination of saikosaponin b2 in Radix Bupleuri oral liquids, nethod：Macroporous absorption resin was employed to purify saikosaponin b2. The HPLC method was performed on a Hypersil C,s column （250 mm ×4. 6 mm ,5μm）with gradient elution of acetonitrile -water（0 - 50 min,30： 70→50： 50） ,the flow rate was 1.0 mL·min^-1 ,the column temperature was set at 30℃,and the detective wavelength was 252 nm. Result：The calibration curve was linear in the range of 0. 03 -0. 30μg （r = 0. 9998 ）for saikosaponin b2. The average recovery（ n = 6 ） was 98.0% （ RSD = 0. 9% ）. Conclusion：This method is sensitive, simple and accurate,and can be used to determine the content of saikosaponin b2 in Radix Bupleuri oral liquids.

关 键 词：柴胡口服液 柴胡皂苷b2 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]