HPLC法同时测定肠激安提取物中3个有效成分的含量  

HPLC determination of three components in Changji’an extract

作　　者：唐洪梅[1] 李得堂[1] 黄樱华[1] 李卓明[2] 

机构地区：[1]广州中医药大学第一附属医院,广州510405 [2]广州中医药大学,广州510405

出　　处：《药物分析杂志》2009年第5期752-754,共3页Chinese Journal of Pharmaceutical Analysis

基　　金：广州市科技局资助项目(2005J1-C0201);广东省医院药学研究基金(2008A016);广东省重大科技专项计划项目(2008AJ-E4101-6)

摘　　要：目的:建立高效液相色谱法同时测定肠激安方中芍药苷、木香烃内酯和去氢木香内酯3个有效成分的含量。方法:采用Phenomenex Luna C18色谱柱(250mm×4.6mm,5μm),以乙腈-水为流动相梯度洗脱,流速0.8mL·min-1,检测波长230nm,柱温30℃。结果:芍药苷、木香烃内酯和去氢木香内酯线性范围分别为0.15～3.03μg(r=0.9999),0.11～2.23μg(r=0.9999),0.10～2.03μg(r=0.9999);平均加样回收率(n=9)分别为100.9%,101.1%,102.0%。肠激安提取物中芍药苷、木香烃内酯和去氢木香内酯的含量分别为(3.38±0.05)mg·g-1,(1.66±0.03)mg·g-1,(1.87±0.02)mg·g-1。结论:本方法的精密度、稳定性、重复性和加样回收率均较好;所建立的HPLC方法准确、快速,可用于肠激安提取物中芍药苷、木香烃内酯和去氢木香内酯3个主要成分的含量测定。Objective ：To establish an HPLC method for determination of three components （peoniflorin, costunolide and dehydro -α -curcumene）in Changji＇an extract. Methods：HPLC analysis was performed on a Phenomenex Luna C18 column（250 mm×4. 6 mm,5μm）with the mixture of acetonitrile and water as mobile phase in gradient elution, and the flow rate was 0. 8 mL·min^-1. The column temperature was 30 ~C, and the detection wavelength was 230 nm. Results：The calibration curves of peoniflorin,costunolide and dehydro -α-curcumene were linear in the ranges of 0. 15 -3.03 μg（r =0. 9999） ,0. 11 -2. 23μg（r=0. 9999） ,0. 10 -2. 03 μg（r =0. 9999） ,respectively;The average recoveries（ n =9）of the three components in Changji＇an extract were 100. 9%, 101.1% and 102. 0% ,respectively. And their contents were （3. 38±0. 05 ） mg·μg^-1, （ 1.66±0. 03 ） mg·g^-1 and （ 1.87±0. 02 ） mg·g^-1. Conclusion： The accuracy, stability, repeatability, and average recovery of the method are satisfying, and the three components （peoniflorin, costunolide and dehydro -α- curcumene）of Changji＇ an extract can be rapidly and accurately quantified by HPLC.

关 键 词：肠激安提取物 有效成分 芍药苷 木香烃内酯 去氢木香内酯 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]