RP-HPLC法测定20(S)-原人参二醇药质体含量及包封率  

Determination of content and entrapment efficiency of 20(S)-protopanaxadiol in pharmacosomes by RP-HPLC method

作　　者：韩美华[1] 陈婧[2] 陈士林[1] 王向涛[1] 

机构地区：[1]中国医学科学院药用植物研究所,北京100193 [2]哈尔滨商业大学生命科学与环境科学研究中心药物研究所抗肿瘤天然药物教育部工程研究中心,黑龙江哈尔滨150076

出　　处：《中国中药杂志》2009年第9期1082-1085,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30600787);中央级公益性科研院所基本科研业务专项药植所重点项目(YZ-1-23)

摘　　要：目的：建立20（S）-原人参二醇药质体含量及包封率的测定方法。方法：采用RP-HPLC法。色谱条件：COSMO-SIL 5C18-MS-Ⅱ色谱柱（4.6 mm×250 mm,5μm）;流动相甲醇-水（95∶5）;流速1.0 mL.min^-1;检测波长203 nm;柱温25℃;进样量50μL;采用低温超速离心法分离PPD药质体中的游离药物。结果：在上述色谱条件下豆磷脂和试剂对药物的测定无干扰,20（S）-原人参二醇在0.1-0.5 g.L^-1与峰面积的线性关系良好（r=0.999 9）,回收率在101.44%-103.11%,日内及日间RSD均小于2%（n=3）。结论：RP-HPLC法可用于20（S）-原人参二醇药质体含量及包封率的测定。Objective： To establish a RP-HPLC method for content and entrapment efficiency of 20 （S）-protopanaxadiol in pharmacosomes. Method： The separation was performed with a COSMOSIL 5 C18-MS-Ⅱ column （4. 6 mm ×250 mm, 5 μm） using methanol-water （95：5 ） as the mobile phase and detected at 203 nm. The flow rate was 1.0 mL .min^ -1 and 50 μL sample solution was injected for each time. Result： The calibration curve was linear within the range 0. 1-0. 5 mg. mL^ -1 （ r = 0. 999 9 ）, the intra-day RSD and inter-day RSD were less than 2% and the average recovery was between 101.44%-103.11% （n = 3）. Conclusion： The method is simple, accurate, sensitive and applicable for determination of content and entrapment efficiency of 20 （S）-protopanaxadiol pharmacosomes.

关 键 词：20(S)-原人参二醇 药质体 包封率 反相高效液相色谱法 

分 类 号：R284[医药卫生—中药学]