冰片对原儿茶酸在家兔体内药动学的影响  

Effects of borneol on pharmacokinetics of protocatechuic acid in rabbits

作　　者：杨洁[1] 王世祥[1] 兰薇[1] 刘春芝[1] 郑晓晖[1] 

机构地区：[1]西北大学生命科学学院,陕西西安710069

出　　处：《中国中药杂志》2009年第9期1141-1143,共3页China Journal of Chinese Materia Medica

基　　金：教育部科学研究重点项目(207151);陕西省中医管理局中医药科研项目(2005103)

摘　　要：目的：研究冰片对原儿茶酸在家兔体内药动学的影响。方法：建立测定家兔血浆中原儿茶酸的高效液相色谱方法。将12只健康新西兰家兔随机分为2组,分别灌胃原儿茶酸（原儿茶酸30 mg.kg^-1）及原儿茶酸-冰片（原儿茶酸30 mg.kg^-1＋冰片15 mg.kg^-1）,采用RP-HPLC法测定家兔给药后不同时间血浆中原儿茶酸的浓度,DAS 2.0药动学软件拟合房室模型,计算药动学参数。结果：原儿茶酸在0.04-2.0 mg.L^-1线性良好（r=0.9983）。原儿茶酸与冰片配伍给药可使原儿茶酸的吸收半衰期（t1/2Ka）明显减小,药物达峰时间（Tmax）显著缩短,血药浓度曲线下面积（AUC（0-∞））增加,表观清除率（CL/F）降低,同时表观分布容积（V1/F）也相应的减小。结论：冰片促进原儿茶酸在家兔体内的吸收,使其吸收量增加,排泄减缓,可提高原儿茶酸的生物利用度。Objective： To study the effect of borneol on the pharmacokinetics of Protocatechuic acid （PA） in rabbits. Method： A method for determinating PA in rabbit plasma by high performance liquid chromatography （HPLC） was established. The 12 healthy New Zealand rabbits were randomly divided into 2 groups. PA concentration in plasma samples of rabbits was determined by HPLC after oral administration of PA （30 mg . kg^-1 ） and PA combined with borneol （protocatechuic acid 30 mg .kg^-1 ＋ bomeol 15 mg .kg^-1 ）. The data were processed by DAS 2.0 software to calculate the pharmaeokinetie parametes. Result： Chromatographic peaks were separated well by the HPLC method. Regression analysis of the data of PA concentration against its peak area showed a good straight line in the range of 0. 04-2. 0 mg.L^-1 （r =0. 998 3）. In comparison with PA alone, the pharmacokinetics parameters of PA （t1/2K,, Tmax, V1/F, CL/F） in the PA ＋ borneol combination group were decreased, while the AUC（0-∞） was increased. Conclusion ： Borneol could significantly promote absorption, increase the dose absorption and slow elimination of PA in rabbits. Borneo1 could improve the bioavailability of PA.

关 键 词：原儿茶酸 冰片 药动学 高效液相色谱 “君-使”对药 

分 类 号：R285.5[医药卫生—中药学]