大肠杆菌麦芽糖结合蛋白(MBP)诱导小鼠Th1细胞的活化作用  

Effect of Escherichia coli maltose-binding protein on mouse Th1 cell activation

作　　者：赵小霞[1] 马吉春[1] 方芳[1] 周静[1] 宋献美[1] 柳忠辉[1] 台桂香[1] 

机构地区：[1]吉林大学白求恩医学院免疫教研室,长春130021

出　　处：《中国免疫学杂志》2009年第6期504-507,共4页Chinese Journal of Immunology

基　　金：吉林省科技发展计划项目资助(20080931)

摘　　要：目的:探讨MBP对小鼠T细胞的调节作用。方法:采用淋巴细胞分层液分离小鼠脾脏淋巴细胞,在体外用MBP刺激淋巴细胞,通过MTT法测定淋巴细胞增殖反应;ELISA检测脾细胞培养上清中细胞因子的分泌水平;MBP免疫小鼠后,ELSPOT检测MBP刺激脾淋巴细胞分泌IFN-γ的细胞频数;免疫组化检测MBP与T细胞的结合作用。结果:MBP以剂量依赖关系促进小鼠淋巴细胞的增殖,促进淋巴细胞分泌IL-2和IFN-γ,轻微抑制IL-4的分泌;ELSPOT检测细胞分泌IFN-γ结果显示,MBP可诱导特异性Th1活化,也可刺激非特异性Th1活化;免疫组化结果显示,抗MBP抗体可与经MBP刺激的淋巴细胞发生反应,阳性细胞占总淋巴细胞的37.7%,当MBP采用1∶2 000抗MBP抗体中和后,再与淋巴细胞反应,结果未见阳性细胞。结论:MBP可作用于淋巴细胞诱导非特异性Th1活化,也可通过免疫诱导大量特异性Th1活化;MBP可作为新的免疫增强剂开发利用。Objective： To study regulatory effect of MBP on mouse T cells. Methods： Lymphocytes were separated from mouse splenocytes by density gradient centrifugation and stimulated with MBP, then its proliferation response was detected by MTT; The levels of cytokines in the culture supernatant of lymphocytes were examined by ELISA; After the mice were immunized with MBP, MBP induced secretion of IFN-γ by specific lymphocytes, which was detected using ELSPOT;The MBP-binding T cells was detected using immunohistochemical staining. Results： The MBP promoted the proliferation of mouse lymphocytes in dose-dependent manner, MBP significantly enhanced secretion of IL-2 and IFN-γ, whereas slightly inhibited secretion of IL-4; It was shown by ELISPOT that MBP could activate both specific and nonspecific Thl cells to secrete IFN-γ; Immunohistochemical staining showed that anti-MBP antibodies reacted with T ceils, which stimulated with MBP beforehand, and positive cell ratio was about 37.7% ;After MBP was neutralized beforehand with anti-MBP antibodies at 1：2 000 dilution,the positive T cells could not nearly be found little. Conclusion： These data suggest that the MBP can not only interact with T cells and stimulate nonspecific Thl activation but also induce specific Thl activation by immunization with MBP, consequentially enhanced cellular immunity. Therefore MBP can be developed as a novel biological response modifier.

关 键 词：麦芽糖结合蛋白 TH1细胞 细胞免疫应答 

分 类 号：R392.11[医药卫生—免疫学]