加味玉屏风散及拆方组分对利什曼原虫感染模型CD4^+CD25^+Treg水平的影响  

Effect of MYP and it's components on CD4^+CD25^+Treg in leishmania infected model

作　　者：刘丹[1] 王莉新[1] 杨秋美[1] 缪珠雷[1] 陈圣敏[1] 王易[1] 

机构地区：[1]上海中医药大学免疫学与病原生物学教研室,上海201203

出　　处：《中国免疫学杂志》2009年第6期517-519,523,共4页Chinese Journal of Immunology

基　　金：上海高校选拔培养优秀青年教师科研专项基金;上海市教委高水平特色发展项目[沪教委财(2005)81号];上海市教委重点学科建设项目(J50301)资助

摘　　要：目的:探讨中药复方加味玉屏风散及拆方组分对感染机体内CD4+CD25+Treg细胞(Treg)潜在的调节作用。方法:选用加味玉屏风散及其拆方单味药组分(黄芪、防风、白术、党参)对利什曼原虫感染模型进行干预。流式细胞仪分析脾细胞内Treg水平;RT-PCR法检测Treg功能相关转录因子Foxp3水平。结果:与正常对照鼠相比较,利什曼原虫感染BALB/c小鼠脾脏内Treg水平和Foxp3 mRNA表达水平均显著升高(P<0.05),复方及其部分单味药可以下调感染动物脾细胞内Treg和Foxp3表达水平(P<0.05),显著抑制感染部位的肿胀程度(P<0.05),减轻病变。结论:利什曼原虫感染BALB/c小鼠存在Treg水平的增加,中药复方加味玉屏风散和部分单味药组分可以显著抑制感染机体内Treg水平,促进感染清除。Objective：To explore the potential regulatory function of the Chinese herb compound Modified YuPingFeng pulvis （MYP） and it＇ s single component onCD4^＋CD25^＋ Treg （Treg） in leishmania-infected models. Methods： The murine modle of leishmania infection was treated with MYP and it＇s single component herbs separately, including Radix astragali （RA）, Radix sileris （ RS ）, Rhizoma Atractylodis Macrocephalae （RAM） and Radix codonopsitis （RC）. The level of Treg in splenocyte was analyzed by FACS; And the expressing level of Foxp3 mRNA, a transcription factor related with the function of Treg, was evaluated with RT-PCR. Results： Compared with those of the control mice, the level of Treg and Foxp3 mRNA increased remarkably in the murine model （ P 〈 0.05）, MYP and some of it＇ s single component herbs could downregulate the level of Treg （ P 〈 0.05） and Foxp3 mRNA （ P 〈 0.05 ） in splenocytes, and inhibited the swelling level in the infected part （P 〈 0.05）. Conclusion：The level of Treg is increased in the leishmania-infected mice and Traditional Chinese herbs MYP compound and some of its single herb components can suppress the level of Treg markedly, whereby to inhibit the development of infectious diseases.

关 键 词：CD4+CD25+调节性T细胞 感染 中药 

分 类 号：R285.5[医药卫生—中药学] R392.5[医药卫生—中医学]