2,5-二-[2-(4-羟基-苯基)乙烯基]吡嗪与白蛋白的相互作用  

A Spectrofluorimetric Study of the Interaction Between 2,5-di-[2-(4-hydroxy-phenyl)ethylene] Pyrazine and Serum Albumin

作　　者：吴芳英[1] 向艳玲[1] 孙梅珍[1] 万筱芬[1] 

机构地区：[1]南昌大学化学系,江西南昌330031

出　　处：《南昌大学学报（理科版）》2009年第2期156-160,共5页Journal of Nanchang University(Natural Science)

基　　金：江西省自然科学基金资助项目(JXNSFNo2007GZH2119);江西省教育厅资助项目(2005-38)

摘　　要：采用荧光光谱法研究了2,5-二-[2-(4-羟基-苯基)乙烯基]吡嗪(1)与蛋白质的相互作用。在pH=7.40的Tris-HCl缓冲溶液中,以406 nm的光激发该化合物在525 nm处有发光,加入人血清白蛋白或牛血清白蛋白后发光增强,其荧光强度与白蛋白浓度之间呈良好的线性关系,线性范围分别为9.1×10-7-1.0×10-6mol.L-1(HSA)和1.1×10-6-8.0×10-6mol.L-1(BSA),检测限分别为9.1×10-8mol.L-1(HSA)和1.1×10-7mol.L-1(BSA)。研究结果表明主体1对白蛋白内源荧光的猝灭源于激发态蛋白质分子与基态的主体1产生分子间的碰撞引起的动态猝灭过程,其猝灭常数分别为1.6×104mol-1.L(HSA)和7.6×103mol-1.L(BSA)。依据For-ster非辐射能量转移理论,确定了供体-受体间的结合距离和能量转移效率。The interaction between fluorescent probe, 2,5 - di - [ 2 - （ 4 - hydroxy - phenyl ） ethylene ] pyrazine （ 1 ）, and serum albumin was studied by fluorescence spectroscopy. 1 emitted weak fluorescence at 525 nm in Tris - HClbuffer solution of pH 7.40 when excitation wavelength was set at 406 nm. The presence of human serum albu- or bovine serum albumin led to remarkable fluorescence enhancement accompanied by the spectral shift. A good linear response of fluorescence intensity as the function of the concentration of serum albumin was obtained. The corresponding linear response ranged from 9.1 ×10-7 to 1.0 ×10-6 mol L-1 for HSA and from 1.1 ×10-6 to 8.0×10-6 mol L -1 for BSA. The detection limits were 9.1 ×10-8 mol L-1 and 1.1×10-7 mol L-1 for HSA and BSA re- spectively. A new method of determining trace amounts of serum albumin was established. The quenching mecha- nism of fluorescence of serum albumin by 1 was investigated to be a dynamic quenching procedure. The quenching constants were 1.6 ×10-4 moL-1 · L（HSA） 和 7.6 ×10- moL · L-1（BSA） ,respectively. The distance and the energy transfer efficiency between 1 and serum albumin were obtained based on the theory of Forester spectroscopy energy transfer.

关 键 词：2 5-二-[2-(4-羟基-苯基)乙烯基]吡嗪 白蛋白 荧光光谱 

分 类 号：O657[理学—分析化学]