超临界流体萃取法测定川芎中藁本内酯含量的研究  

THE DETERMINATION OF LIGUSTILIDE IN LIGUSTICUM CHUANXIONG HORT. BY SUPERCRITICAL FLUID EXTRACTION

作　　者：吴广通[1,2] 石力夫[1,2] 胡晋红[1,2] 李玲[1,2] 

机构地区：[1]第二军医大学附属长海医院药学部 [2]第二军医大学药学系药分教研室

出　　处：《药学学报》1998年第6期457-460,共4页Acta Pharmaceutica Sinica

摘　　要：系统研究了压力、温度、静态萃取时间、动态萃取量、改性剂加入量等因素对超临界流体萃取（ＳＦＥ）中药川芎中藁本内酯的影响，确定最佳萃取条件为：压力２７６ＭＰａ，温度４０℃，静态萃取时间３ｍｉｎ，动态萃取量７ｍｌ，改性剂加入量０１ｍｌ。并对超临界流体萃取的收集方法作了研究，发现固液收集法在收集效率和精密度方面比溶剂收集法效果好。并用离线的ＳＦＥＲＰＨＰＬＣ对川芎药材中藁本内酯的含量进行了测定。In this study, a systematic method was used to optimize the supercritical fluid extraction (SFE) of ligustilide in Ligusticum chuanxiong Hort. Overall five variables (pressure, temperature, static extracting time, modifier concentration and CO2 dynamic extracting volume) were considered. To reduce the loss of volatile substance, a solidliquid trap was developed for SFE collection. Comparisons were made on the solvent trap for SFE. The extracts were analyzed by high performance liquid chromatography (HPLC) with ultraviolet absorbance detection. The recoveries of 9934% (RSD 170%) and 9211% (RSD 572%) were achieved for solidliquid trap and solvent trap, respectively.SFE conditions: temperature 40℃, pressure 276 MPa, static extraction 3 min, dynamic extraction 7 ml and 01 ml CHCl3 as modifier.HPLC conditions: The HPLC column (39 mm×150 mm)was packed with NovaPak C18 (4 μm). Naphthalene was used as internal standard. The mobile phase was methanol—10% isopropyl alcohol solution (53∶47 v/v). The detection wavelength was 280 nm and the flow rate was 08 ml·min-1.

关 键 词：超临界萃取 川芎 藁本内酯 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]