FⅦa依赖TF及FⅩ活化PAR2上调SW 620细胞IL-8表达  

Activation of Protease-Activated Receptor 2 by FⅦa Induced IL-8 Expression in SW 620 Cells Depending on TF and FX

作　　者：石文霞[1] 周红[1] 李娜[1] 黄宏亮[1] 周保成[1] 

机构地区：[1]江苏大学基础医学与医学技术学院,镇江212013

出　　处：《血栓与止血学》2009年第2期53-56,共4页Chinese Journal of Thrombosis and Hemostasis

摘　　要：目的探讨凝血FⅦa、Ⅹ、Ⅱa在蛋白酶激活受体2(PAR2)活化及诱导SW620细胞IL-8表达中的作用。方法用PAR2激动剂(PAR2-AP)、FⅦa、Ⅹ、Ⅱa等不同刺激物处理SW620细胞,以ELISA法检测细胞上清IL-8水平,以实时定量PCR检测细胞IL-8mRNA表达。结果血浆浓度的Ⅶa需在FⅩ存在下才能促进细胞IL-8表达;单克隆抗TF及抗PAR2抗体均可抑制FⅦa的作用;FⅡa轻微下调细胞IL-8表达,Hirudin、抗PAR1及抗PAR2抗体均可阻断此作用。结论TF-FⅦa及TF-FⅦa-FⅩa复合物,而非FⅡa,活化PAR2上调SW620细胞IL-8表达,从而促进细胞增殖及迁移。Objective To investigate the roles of FⅦa, X and Ⅱ a on protease-activated receptor-2 activation and IL- 8 expression in SW 620 cells. Methods The effects of PAR2- AP, FⅦ a, X,Ⅱ a on IL- 8 protein and mRNA expression in SW 620 cells were analysed by ELISA and QT-PCR. Results Plasma concentration of FⅦa combined with F X could induce IL- 8 expression in SW 620 cells. The effects of FⅦa could be blocked not only by anti- TF but also by anti- PAR2 antibody. F Ⅱ a showed slightly downregulation on IL-8 expression, and could be attenuated with Hirudin, anti-PAR1 or anti-PAR2 antibody. Conclusion TF-FⅦa and TF-FⅦa-FXa complex, but not FⅡa, may activate PAR2 and then induce IL-8 expression in SW 620 cells, which promotes the growth and migration of cancer cells.

关 键 词：蛋白酶激活受体2(PAR2) 因子Ⅶa 白介素-8(IL-8) 结肠癌细胞株SW 620 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]