血管紧张素Ⅱ对新生大鼠脑星形胶质细胞组织因子表达的影响  

Effect of Angiotensin Ⅱ on Tissue Factor Expression in Rat Astrocytes

作　　者：何美霞[1] 傅蔓华[2] 华海婴[1] 叶启霞[1] 戈士文[1] 朱建立[1] 李月白[3] 贺石林[4] 

机构地区：[1]郑州大学,医药科学研究院,河南省医药科学研究院,郑州450052 [2]河南省中医药研究院,郑州450004 [3]郑州大学,基础医学院生物化学与分子生物学教研室,郑州450052 [4]中南大学,湘雅医学院,生理学系止血生理研究室,长沙410078

出　　处：《血栓与止血学》2009年第1期5-7,共3页Chinese Journal of Thrombosis and Hemostasis

基　　金：河南省科技厅资助项目(No.0441130302)

摘　　要：目的观察血管紧张素Ⅱ(AngⅡ)对新生大鼠脑星形胶质细胞组织因子(TF)表达的影响及机制。方法培养Wistar新生大鼠脑星形胶质细胞。细胞促凝活性(PCA)的检测采用一期凝固法;细胞TF抗原测定采用ELISA法。结果AngⅡ(10-9～10-7M)可剂量依赖性诱导新生大鼠脑星形胶质细胞PCA、TF抗原的增加,并有明显的时效关系。洛沙坦(losartan)浓度在10-6～10-5M可不同程度地阻断AngⅡ的作用。结论AngⅡ可诱导新生大鼠脑星形胶质细胞组织因子的表达,该作用是通过血管紧张素Ⅱ1型受体实现的。Objective To elucidate whether angiotensin Ⅱ （Ang Ⅱ ） induced astrocytes to express tissue factor （TF）. Methods Astroeytes were isolated from neonatal Wistar rats. Procoagulant activity （PCA） was determined with one-stage clotting method. TF antigen was assayed by ELISA. Results Ang Ⅱ （10^-9 -10^-7M） induced significantly astrocytes to increase PCA,TF antigen in a dose and time dependent manner; Losartan （10^-6- 10^-5M） significantly inhibited the stimulative effects of Ang Ⅱ on TF activity, antigen, and the dose-dependent effects was observed. Conclusion The present data suggested that Ang Ⅱ could induce the expression of TF in rat astrocytes, and this effect was mediated by AT1R.

关 键 词：星形胶质细胞 血管紧张素Ⅱ 组织因子 

分 类 号：Q461[生物学—生理学]