A new fluorescent particle prepared by chemical stabilized phycobilisome  

作　　者：Min Chen Guo Ping Ma Li Sun 

机构地区：[1]College of Chemical and Biological Sciences and Engineering, Yantai University, Shandong 264005, China [2]The Clinic, Naval Aeronautical Engineering Institute, Shandong 264001, China

出　　处：《Chinese Chemical Letters》2009年第6期724-728,共5页中国化学快报（英文版）

基　　金：by Prize Fund for Excellent Middle Aged and Young Scientists of Shandong Province(No.2006BS02016);National Science Fund(No.30571720)

摘　　要：Natural phycobilisomes （PBSs） were isolated and purified from a red macroalga, Polysiphonia urceolata, by multi-step of sucrose gradient centrifugation, and were chemically stabilized by small molecule cross-linker formaldehyde. The stabilized PBSs showed similar absorption and fluorescent properties at room temperature compared to natural PBSs and kept a steady F672/F580 value during more than 3 months of storage in 0.45 mol/L phosphate buffer （pH 6.8） or at low temperature at 77 K. The stabilized PBS migrated as a single band at mild PAGE and in 14-18 h of sucrose gradient centrifugation. All these characters indicated that the stabilized PBSs were stable, soluble, homogenous fluorescent particles with favorable spectroscopic features prepared under present conditions.Natural phycobilisomes （PBSs） were isolated and purified from a red macroalga, Polysiphonia urceolata, by multi-step of sucrose gradient centrifugation, and were chemically stabilized by small molecule cross-linker formaldehyde. The stabilized PBSs showed similar absorption and fluorescent properties at room temperature compared to natural PBSs and kept a steady F672/F580 value during more than 3 months of storage in 0.45 mol/L phosphate buffer （pH 6.8） or at low temperature at 77 K. The stabilized PBS migrated as a single band at mild PAGE and in 14-18 h of sucrose gradient centrifugation. All these characters indicated that the stabilized PBSs were stable, soluble, homogenous fluorescent particles with favorable spectroscopic features prepared under present conditions.

关 键 词：Polysiphonia urceolata PBSs Chemical stabilization FORMALDEHYDE 

分 类 号：O614.33[理学—无机化学] TB332[理学—化学]