益气活血化瘀中药对大鼠前列腺增生组织血管新生及bFGF表达的影响  

Effects of Yiqi Huoxue Huayu Chinese medicine on expression of bFGF and neogenesis of blood vessels in BPH rats

作　　者：杨欣[1] 张永华[1] 丁彩飞[1] 颜志中[1] 倪卫东[1] 

机构地区：[1]浙江省杭州市红十字会医院,杭州310003

出　　处：《中华中医药杂志》2009年第6期780-783,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：浙江省中医药科技计划项目(No.2006C110);杭州市科技发展计划项目(No.20070433Q02)

摘　　要：目的:观察益气活血化瘀中药对大鼠前列腺增生组织血管新生及碱性成纤维细胞生长因子(bFGF)的影响。方法:50只SD大鼠去势7d后,皮下注射丙酸睾酮5mg.kg-1.d-1,同时给予益气活血化瘀方、泽桂癃爽混悬液灌胃,30d后股动脉放血处死大鼠,摘取前列腺,测量前列腺体积、计算前列腺指数(PI)、检测前列腺微血管密度(MVD)和前列腺组织中bFGF蛋白表达。结果:与正常组比较,模型组大鼠前列腺体积、指数均明显增大(P<0.01),前列腺组织MVD明显增高(P<0.01),bFGF蛋白表达显著增高(P<0.01);各给药组前列腺体积、前列腺指数、MVD、bFGF蛋白表达均明显低于模型组(P<0.05或P<0.01);益气化瘀中、高剂量组前列腺体积、前列腺指数、MVD、bFGF蛋白表达均明显低于泽桂癃爽组(P<0.05)。结论:益气活血化瘀中药抗前列腺增生的作用机制与其抑制bFGF过度表达,进而抑制前列腺增生组织新生血管形成有关。Objective： To study the effects of Yiqi Huoxue Huayu Chinese medicine on neogenesis of blood vessels and basic fibroblast growth factor （bFGF） expression in benign prostatic hyperplasia （BPH） rats. Methods： 7 days after being castrated, fifty SD rats were injected with testosterone （5 mg·kg^-1·d^-1） ,the decoction of Yiqi Huoxue Huayu and Zerguilongshuang were orally administrated respectively, after 30 days＇ treatment, the rats were sacrificed, the prostate volume were measured, and the prostate index（PI） were analyzed, the micro-vessels density（MVD） and level of bFGF were measured. Results： The prostate volume and index of rats in model group was obviously higher than those in normal group （ P〈0.01 ） , and the levels of MVD and bFGF in model group obviously increased （ P〈0.01 ） as compared with nornal group; The prostate volume, index, levels of MVD and bFGF in all treatment groups were remarkably lower than those in the model group （ P〈0.05 or P〈0.01 ） . The prostate volume, index, levels of MVD and bFGF in the middle and high doses of Yiqi Huoxue Huayu Chinese medicine groups remarkably decreased as compared with the Zerguilongshuan group （ P〈0.05 ） . Conclusion： Yiqi Huoxue Huayu could inhibit BPH, the mechanisms may be related to restrain the levels of bFGF, and renew of blood vessel.

关 键 词：前列腺增生 大鼠 血管新生 碱性成纤维细胞生长因子 益气活血化瘀 

分 类 号：R285.5[医药卫生—中药学]